中南医学科学杂志2025，Vol.53Issue(2)：236-240,5.DOI:10.15972/j.cnki.43-1509/r.2025.02.010
七氟醚调控HIF-1信号通路对糖尿病大鼠心肌IR损伤的保护作用
Protective effects of sevoflurane on myocardial ischemia-reperfusion injury in diabetic rats by regulating HIF-1 signaling pathway
摘要
Abstract
Aim To investigate the protective effects of sevoflurane on myocardial ischemia-reperfusion(IR)injury in dia-betic rats by regulating HIF-1 signaling pathway.Methods Male diabetic rats were divided into sham operation group,model group,sevoflurane group,2ME2 group(HIF-1 inhibitor),and 2ME2+sevoflurane group.Except the sham operation group,the other four groups were used to establish diabetic myocardial IR injury models.Cardiac function,myocardial infarction area,autopha-gosome number,and the expression levels of HIF-1α,BNIP3,LC3BⅡ/LC3BⅠ,and Beclin-1 in myocardial tissue were measured.Results Compared with the sham operation group,the myocardial infarction area and autophagosome number in the other four groups were increased,and the sevoflurane group was lower than the model group,while the 2ME2 group and 2ME2+sevoflurane group were higher than the sevoflurane group(P＜0.05).Compared with the sham operation group,left ventricular ejection fraction and fraction-al shortening in the other four groups were decreased,and the sevoflurane group was higher than the model group,while the 2ME2 group and 2ME2+sevoflurane group were lower than the sevoflurane group(P＜0.05).Compared with the sham operation group,the expression levels of HIF-1α,BNIP3,LC3BⅡ/LC3BⅠ,and Beclin-1 proteins in the other four groups were increased(P＜0.05).The sevoflurane group had higher HIF-1α and BNIP3 protein levels than the model group,while LC3BⅡ/LC3BⅠ and Beclin-1 protein levels were lower than the model group.The 2ME2 group and 2ME2+sevoflurane group had lower HIF-1α and BNIP3 protein levels than the sevoflurane group,while LC3BⅡ/LC3BⅠ and Beclin-1 protein levels were higher than the sevoflurane group(P＜0.05).Conclusion Sevoflurane improves myocardial IR injury in diabetic rats by upregulating the HIF-1 signaling pathway.关键词
低氧诱导因子-1/糖尿病/大鼠/七氟醚/心肌缺血再灌注/线粒体自噬Key words
HIF-1/diabetes/rats/sevoflurane/myocardial ischemia reperfusion/mitophagy分类
医药卫生引用本文复制引用
杨华,康雅琴,李娟..七氟醚调控HIF-1信号通路对糖尿病大鼠心肌IR损伤的保护作用[J].中南医学科学杂志,2025,53(2):236-240,5.基金项目
荆州市科技计划项目(20230193) （20230193）
