农业生物技术学报2025，Vol.33Issue(5)：1044-1052,9.DOI:10.3969/j.issn.1674-7968.2025.05.009
湖羊NR4A1基因3'UTR区多态性及其与早期生长性状的关联分析
Polymorphisms in the 3'UTR of NR4A1 Gene and Its Association Analysis with Early Growth Traits in Hu Sheep(Ovis aries)
摘要
Abstract
Nuclear receptor subfamily 4 group A member 1(NR4A1)is widely expressed in tissues of sheep(Ovis aries)and plays multiple biological functions,participating in the regulation of muscle and bone development.This study aimed to investigate the sequence characteristics and polymorphisms in the 3'untranslated regions(3'UTR)of NR4A1 and its correlation with early growth traits in Hu sheep.The 3'UTR sequence of NR4A1 was obtained by PCR,rapid amplification of cDNA ends(RACE)and Sanger sequencing,and analyzed by bioinformatics method.The mutation sites in NR4A1 3'UTR were screened by Pooled-DNA sequencing,and genotyped by DNA sequencing.Then,the polymorphism of the sequence and its correlation with early growth traits was analyzed.Results showed that NR4A1 3'UTR sequence of Hu sheep with a length of 556 bp was obtained.Two mutations,g.26473 C＞T and g.26656 G＞A,were found in the 3'UTR of NR4A1 of Hu sheep.The 2 loci were completely linked with 3 genotypes,named CCGG,CTAG and TTAA,and CCGG was the dominant genotype(88.4％).Association analysis showed that there was no significant difference in birth weight among the 3 genotypes(P＞0.05),while weaning weight and average daily gain from birth to weaning with CTAG genotype were significantly higher than those with CCGG genotype in Hu sheep(P＜0.05).In conclusion,the 3'UTR of NR4A1 of Hu sheep has 2 completely linked mutation sites with 3 genotypes CCGG,CTAG and TTAA,and Hu sheep with CTAG genotype in the 3'UTR of NR4A1 has advantages in early growth,which is expected to become a potential marker for the molecular breeding of sheep.关键词
湖羊/核受体亚家族4 A组成员1(NR4A1)/非翻译区3'端(3'UTR)/多态性/生长性状Key words
Hu sheep/Nuclear receptor subfamily 4 group A member 1(NR4A1)/3'untranslated regions(3'UTR)/Polymorphism/Growth traits分类
农业科技引用本文复制引用
李晓彤,于昊,王悦,谢婧,茆达干..湖羊NR4A1基因3'UTR区多态性及其与早期生长性状的关联分析[J].农业生物技术学报,2025,33(5):1044-1052,9.基金项目
国家重点研发计划项目(2022YFD1300205) （2022YFD1300205）
