山东农业大学学报（自然科学版）2025，Vol.56Issue(2)：285-292,8.DOI:10.3969/j.issn.1000-2324.2025.02.011
苹果NCED基因家族鉴定及表达分析
Identification and Expression Analysis of Apple NCED Gene Family
摘要
Abstract
Abscisic acid(ABA)plays a crucial role when plants are coping with abiotic stresses.NCED exerts important regulatory effects in the synthesis of ABA in organisms.This research analyzes the members of apple NCED gene family through bioinformatics methods,then detects the expression of apple MdNCEDs under different drought and salt stress conditions using expression profiles.The results show that 18 MdNCED gene family members are identified in this study.Chromosome localization analysis indicates that these members are distributed on 10 chromosomes.The molecular weight of MdNCEDs is 46.47-76.36 kD,and the isoelectric point(pI)is between 5.2-7.63.A variety of functional elements relates to stress hormones and responses to abiotic stress are detected in apple MdNCED promoter sequence.Gene structure analysis reveals that the structure of MdNCED gene is highly consistent.The results of collinearity analysis demonstrat that duplication events occur between some genes.The expression data shows that compared with CK(0 h),three genes(MdMYB3,MdMYB11,MdMMYB18)are up-regulated,and four genes(MdMYB1、MdMYB6、MdMYB13、MdMYB17)are down-regulated under high-salt and drought stress conditions.There is no obvious regularity in the expression of MdMYB4 and MdMYB14 or significant differences in the four genes(MdMYB5,MdMYB8,MdMYB9,MdMYB16).This paper provides a basic theoretical basis for further analysis of the function of MdNCED genes in apple.关键词
苹果/脱落酸/NCED基因家族/生物信息学分析Key words
Apple/abscisic acid/NCED gene family/Bioinformatics analysis分类
农业科技引用本文复制引用
李嘉伟,徐佳灿,崔建华,王欣,刘卓,张旭珠,吴瑞刚..苹果NCED基因家族鉴定及表达分析[J].山东农业大学学报（自然科学版）,2025,56(2):285-292,8.基金项目
河北省自然基金面上项目(C2022402006) （C2022402006）
邯郸市科技计划项目(21422012321) （21422012321）
