山东农业大学学报（自然科学版）2025，Vol.56Issue(2)：353-361,9.DOI:10.3969/j.issn.1000-2324.2025.02.019
巨大芽孢杆菌F1配施有机物料对土壤理化性质的影响
Bacillus megaterium F1 Combined with Organic Materials on Soil Physical and Chemical Properties
摘要
Abstract
Soil health is critical for ensuring food security and sustainable agricultural development.The quantity and quality of soil aggregates are key indicators for evaluating soil health.In order to investigate the effects of co-application of extracellular polysaccharide-producing Bacillus megaterium F1 and organic materials on soil aggregates,soil nutrients,and its potential for soil improvement and application,F1 fermentation broth,organic materials,and their combination are applied separately to soil for cultivation.A soil aggregate analyzer is used to assess changes in the content of water-stable aggregates.Soil alkaline hydrolyzable nitrogen,available phosphorus,available potassium,organic matter,and soil polysaccharides are determined using the alkaline diffusion method,molybdenum-antimony anti-spectrophotometry,flame atomic absorption spectrophotometry,potassium dichromate dilution method,and anthrone sulfate colorimetric method,respectively.Principal component analysis(PCA)is employed to comprehensively evaluate the soil improvement effects of F1 fermentation broth and organic materials.The results show that F1 fermentation broth significantly promotes soil aggregate formation,and the combined application of F1 fermentation broth with organic materials enhances both soil fertility and the formation and stability of soil aggregates.After 42 days of cultivation,the treatment with F1 fermentation broth and different organic materials increase the total content of water-stable aggregates by 41.08%～41.80%,reduce the fractal dimension by 14.55%～15.32%,and raise the levels of soil alkaline nitrogen by 12.09%～38.25%,available phosphorus by 88.17%～241.10%,available potassium by 74.79%～421.06%,organic matter by 53.15%～108.01%,and polysaccharides by 164.93%～170.60%.PCA reduces seven indicators to three principal components,explaining 88.49%of the variance in the original data.The combined application of F1 fermentation broth and Flammulina velutipes residue notably increase the content of 0.25-5 mm water-stable aggregates,soil structure and aggregate stability,and improve the levels of available nutrients and organic matter,demonstrating the most significant soil improvement effect.关键词
水稳性团聚体/土壤养分/胞外多糖/巨大芽孢杆菌/有机物料Key words
Water stable aggregates/soil nutrients/extracellular polysaccharides/Bacillus megaterium/organic materials分类
农业科技引用本文复制引用
吴瑜健,马海林,孙姗姗,成龙,杨新宇,张明泽,丁延芹..巨大芽孢杆菌F1配施有机物料对土壤理化性质的影响[J].山东农业大学学报（自然科学版）,2025,56(2):353-361,9.基金项目
山东省重点研发计划(重大科技创新工程)项目(2021CXGC10804) （重大科技创新工程）
