水产学报2025，Vol.49Issue(5)：20-28,9.DOI:10.11964/jfc.20240514536
圆斑星鲽雌性特异DNA分子标记开发及遗传性别鉴定方法的建立
Identification of a female-specific DNA marker and development of genetic sex identification method in Verasper variegatus
摘要
Abstract
Verasper variegatus,a marine flatfish,holds significant nutritional and economic value and exhibits sexual dimorph-ism in growth,with females growing faster than the males.However,to date,no rapid,effective,and universal sex-specific DNA marker has been available for genetic sex identification and sex-controlled breeding in V.variegatus.To address this,we performed whole genome resequencing on 17 male and 17 female individuals to identify female-specific DNA sequences.Through PCR amplification,we identified a female-specific DNA marker and established a genetic sex identification method.Our results revealed 359 potential female-specific DNA sequences following reads mapping,sequencing depth,and coverage analysis.Primer pairs targeting approximately 150 bp DNA sequences flanking these regions were designed.After two rounds of PCR verification,a 69 bp insertion was identified as a female-specific marker,accurately distinguishing genetic sex via PCR amplification and 2％agarose gel electrophoresis.Further validated using 117 individuals from another breeding population confirmed the accuracy of this marker and method,with genetic sex results aligning with phenotypic determined by gonad his-tology.The development of this female-specific DNA marker and the genetic sex identification method provides crucial genetic insights into the sex determination mechanism of V.variegatus and accelerates the implementation of sex-controlled breeding and all-female breeding techniques.关键词
圆斑星鲽/雌性特异DNA分子标记/遗传性别鉴定/基因组重测序Key words
Verasper variegatus/female-specific DNA marker/genetic sex identification/genome resequencing分类
水产学引用本文复制引用
郑卫卫,陈松林,徐文腾,刘洋,陈亚东,杨涛,席晓晴,刘志鸿,徐东,秦搏..圆斑星鲽雌性特异DNA分子标记开发及遗传性别鉴定方法的建立[J].水产学报,2025,49(5):20-28,9.基金项目
科技创新2030—重大项目(2023ZD0405505) （2023ZD0405505）
山东省重点研发计划项目(创新平台)(2022CXPT002) （创新平台）
中央级公益性科研院所基本科研业务费专项(2023TD19) （2023TD19）
山东省泰山学者攀登计划 （）
中国水产科学研究院东海水产研究所基本科研业务费专项(2016M08) STI2030-Major Projects(2023ZD0405505) （2016M08）
Shandong Key R & D Program(2022CXPT002) （2022CXPT002）
Central Pub-lic-interest Scientific Institution Basal Research Fund,CAFS(2023TD19) （2023TD19）
Taishan Scholar Climbing Project of Shandong Province （）
Central Public-interest Scientific Institution Basal Research Fund,ECSFRI,CAFS(2016M08) （2016M08）
