水产学报2025，Vol.49Issue(5)：41-49,9.DOI:10.11964/jfc.20220613526
外源的微藻DGAT基因表达对酿酒酵母生长和脂质的影响
Effects of expressing exogenous microalgal DGAT genes on the growth and lipids of Saccharomyces cerevisiae
摘要
Abstract
The H1246 mutant of Saccharomyces cerevisiae,which is deficient in triacylglycerol(TAG)synthesis,is commonly used to elucidate the functions of exogenous genes encoding diacylglycerol acyltransferase(DGAT).To investigate the effects of exogenous DGATs on lipid synthesis and yeast cell growth,one type Ⅰ(DGAT1)and three type Ⅱ DGAT(DGAT2a,DGAT2b,DGAT2c)genes of Myrmecia incisa were individually transformed into the H1246 strain,yielding four transgenic yeast strains.The morphology,lipid droplet formation,cell density,volumetric biomass,biomass productivity,total lipids,total fatty acids,and TAG content of S.cerevisiae were analyzed using microscopic observation,spectrophotometry,gravimetry,Rod thin-layer chromatography,and gas chromatography-mass spectrometry.Microscopic observation during the stable growth phase revealed oil droplets in all four transgenic strains,indicating that the exogenous genes promoted TAG synthesis and stor-age.Lipid component analysis showed that the tDGAT1 strain exhibited the highest TAG and fatty acid content among all the transgenic strains and the wild-type Scy62.Growth performance analysis revealed that cell densities of strains transformed with the exogenous genes reached the levels comparable to Scy62 yeast,likely due to the consumption of free fatty acids during TAG synthesis,thereby reducing cellular damage.However,the tDGAT1 strain exhibited the lowest growth,attributed to a significantly prolonged period of growth retardation.Differences in growth performance and lipid components among the transgenic strains may be due to unique domains with the DGATs.This study provides a foundation for producing desired lip-ids using genetically engineered yeast strains.关键词
酿酒酵母/缺刻缘绿藻/二酰基甘油酰基转移酶(DGAT)/三酰甘油(TAG)/生长Key words
Saccharomyces cerevisiae/Myrmecia incisa/diacylglycerol acyltransferase(DGAT)/triacylglycerol(TAG)/growth分类
水产学引用本文复制引用
胡澄溪,刘伟,毕燕会,周志刚..外源的微藻DGAT基因表达对酿酒酵母生长和脂质的影响[J].水产学报,2025,49(5):41-49,9.基金项目
国家自然科学基金(31772821) （31772821）
国家"双一流"水产学科 National Natural Science Foundation of China(31772821) （31772821）
National"Double First-class"Aquatic Science （）
