水产学报2025，Vol.49Issue(5)：50-57,8.DOI:10.11964/jfc.20230614048
急性碱度胁迫对中华绒螯蟹抗应激、抗氧化能力和肌肉组织结构的影响
Effects of acute alkalinity stress on anti-stress,antioxidant capacity and muscle tissue structure of Eriocheir sinensis
摘要
Abstract
China possesses abundant saline-alkali water resources that are currently underutilized.Developing saline-alkali water aquaculture could transform these abandoned resources into valuable assets and enhance fishery production.This study aimed to investigate the effects of acute alkalinity stress on the physiological metabolism of Eriocheir sinensis.A control group(0 mmol/L)and an alkalinity group(60 mmol/L)were established,with four treatment times(0,6,12,and 24 h)to examine the impacts of acute alkalinity stress on the mRNA expression of anti-stress and developmental regulatory genes(mTOR and AKT)in the hepatopancreas of E.sinensis,as well as antioxidant capacity indicators[catalase(CAT),total antioxidant capacity(T-AOC),malondialdehyde(MDA)]and muscle tissue structure,which are key tissues for energy metabolism.Results showed that after 6 h of alkalinity stress,AKT gene expression increased and then decreased,but remained significantly higher than the con-trol group at 24 h(P＜0.05).mTOR expression significantly increased after 6 h of stress(P＜0.05)and remaind highly expressed.CAT enzyme activity in the hepatopancreas of E.sinensis significantly decreased after 6 h of stress(P＜0.05).T-AOC levels in the hepatopancreas significantly increased after 24 h of alkalinity stress(P＜0.05),while MDA content signific-antly increased with prolonged stress,peaking at 24 h(P＜0.05).Muscle tissue structure in E.sinensis was not significantly affected by 6 h of alkalinity stress.The observed increases in antioxidant enzyme activity(T-AOC)and upregulation of stress-resistant and developmental genes(AKT and mTOR)indicated that E.sinensis actively responded to alkalinity stress.This study provides theoretical guidance for eluciating the anti-stress mechanisms of E.sinensis in saline-alkali environments and for the future development of E.sinensis aquaculture.关键词
中华绒螯蟹/碱度胁迫/肝胰腺/抗氧化能力/抗应激Key words
Eriocheir sinensis/alkalinity stress/hepatopancreas/antioxidant capacity/anti-stress分类
水产学引用本文复制引用
陈涵,邱启勇,王美垚..急性碱度胁迫对中华绒螯蟹抗应激、抗氧化能力和肌肉组织结构的影响[J].水产学报,2025,49(5):50-57,8.基金项目
江苏省种业振兴揭榜挂帅项目[JBGS(2021)031] （2021）
中国水产科学研究院基本科研业务费专项(2023TD39) （2023TD39）
国家重点研发计划(2022YFD2400702) （2022YFD2400702）
淡水渔业研究中心基本科研业务费专项(2023JBFM05) Jiangsu Revitalization of Seed Industry(JBGS[2021]031) （2023JBFM05）
Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2023TD39) （2023TD39）
National Key R & D Program of China(2022YFD2400702) （2022YFD2400702）
Central Public-interest Scientific Institution Basal Research Fund,Freshwater Fisheries Research Center,CAFS(2023JBFM05) （2023JBFM05）
