上海针灸杂志2025，Vol.44Issue(4)：482-489,8.DOI:10.13460/j.issn.1005-0957.2025.04.0482
雀啄灸对湿热痹阻型类风湿关节炎大鼠滑膜抗瓜氨酸化蛋白抗体免疫复合物表达的影响
Effect of sparrow-pecking moxibustion on the expression of anti-citrullinated protein antibodies-immune complex in the synovium of rats with rheumatoid arthritis of damp heat stasis pattern
摘要
Abstract
Objective To observe the effect of sparrow-pecking moxibustion on the expression of anti-citrullinated protein antibodies-immune complex(ACPA-IC)in the synovium of rats with rheumatoid arthritis(RA)of damp heat stasis pattern and to discover the immune mechanism of moxibustion intervening in early-stage RA and the modern biological mechanism of moxibustion treating heat patterns.Method Eighteen rats were randomly selected from 72 female Sprague-Dawley(SD)rats as a blank control group,and the other 54 rats were prepared to be disease-pattern-combined RA models of damp heat stasis pattern using a combo modeling method(collagen-induced arthritis plus environmental stimuli,including wind,damp,and heat).The model was assessed using the arthritis index(AI)and indicators of heat and pain patterns.The successful rat models were randomized into a model group,a medication group,and a sparrow-pecking moxibustion group,with 18 rats in each group.Rats in the medication group received methotrexate suspension via gavage,and rats in the sparrow-pecking moxibustion group received sparrow-pecking moxibustion.When the intervention finished,the AI score,joint surface temperature,and plantar thermal pain threshold value were collected from each group.The ultrastructure of synovial cells of the four groups of rats was observed using transmission electron microscopy;the levels of interleukin-1(IL-1)and tumor necrosis factor-α(TNF-α)in the synovium of bilateral knee joints were detected using the enzyme-linked immunosorbent assay(ELISA);the expression of synovial ACPA-IC was determined using Western blotting.Result Compared to the blank control group,the plantar thermal pain threshold of the diseased joint side declined in the model group rats(P＜0.05);the model group also presented synovial cell swelling,inflammatory changes in the nucleus and mitochondria,and increased AI scores,joint surface temperatures,and synovial IL-1,TNF-α,and ACPA-IC levels(P＜0.05).Compared to the model group,rats in the medication and sparrow-pecking moxibustion groups showed increased plantar thermal pain thresholds(P＜0.05),reduced synovial cell swelling,improved inflammatory changes in the nucleus and mitochondria,and decreased AI scores,joint surface temperatures,and synovial IL-1,TNF-α,and ACPA-IC levels(P＜0.05).Compared to the medication group,the plantar thermal pain threshold was higher(P＜0.05),and the levels of synovial IL-1,TNF-α,and ACPA-IC were lower in the sparrow-pecking moxibustion group rats(P＜0.05).Conclusion Sparrow-pecking moxibustion can improve inflammatory injuries in the synovial cells,reduce joint surface manifestations of heat and pain,regulate synovial ACPA-IC levels,and down-regulate synovial IL-1 and TNF-α levels in rats with RA of damp heat stasis pattern.关键词
灸法/艾条灸/雀啄灸/关节炎,类风湿/湿热痹阻/特异性免疫复合物/大鼠Key words
Moxibustion/Moxa stick moxibustion/Sparrow-pecking moxibustion/Arthritis,Rheumatoid/Damp heat stasis/Specific immune complex/Rats分类
医药卫生引用本文复制引用
邓慧榕,岳婷,杨东宇,刘煜,王煜,杨吉勃,赵中亭,严兴科,朱田田..雀啄灸对湿热痹阻型类风湿关节炎大鼠滑膜抗瓜氨酸化蛋白抗体免疫复合物表达的影响[J].上海针灸杂志,2025,44(4):482-489,8.基金项目
国家自然科学基金地区基金项目(82060892,81560791) （82060892,81560791）
甘肃省科技厅省自然基金项目(20JR10RA313) （20JR10RA313）
甘肃省重点人才项目(2024年) （2024年）
