山东科学2025，Vol.38Issue(2)：73-79,7.DOI:10.3976/j.issn.1002-4026.20240128
碳氮元素数量比对典型耐盐微生物增殖影响
Effects of carbon-to-nitrogen ratio on the proliferation of typical salt-tolerant microorganisms
摘要
Abstract
Bacillus velezensis,Bacillus thuringiensis and Brevibacterium frigoritolerans are three typical salt-tolerant plant growth-promoting rhizobacteria(ST-PGPR)known for their ability to alleviate salt stress in plants.To optimize the nutritional conditions for the growth and proliferation of these strains,experiment of single factor method was used to investigate the effects of different carbon-to-nitrogen ratios(nc/nN)on their development.The results showed that all three strains could grow and reproduce in a medium with a nc/nN of 3.25～6.00.However,the nc/nN had varying effects on their proliferation,i.e.,the most significant impact was observed on the proliferation of velezensis,followed by thuringiensis and frigoritolerans.When the nc/nN was 4∶1,velezensis exhibited the highest proliferation,with a viable bacterial count of 6.2 × 108CFU/mL.Similarly,thuringiensis achieved its highest proliferation at a nc/nN of 4∶1,with a viable bacterial count of 5.1 × 108 CFU/mL.Conversely,frigoritolerans achieved its highest proliferation at a nc/nN of 5∶1,with a viable bacterial count of 3.5 × 108 CFU/mL.In conclusion,the optimal nc/nN for the proliferation of velezensis and thuringiensis is 4∶1,whereas that for frigoritolerans is 5∶1.关键词
耐盐微生物/碳氮比/培养基优化/微生物生物量Key words
salt-tolerant microorganisms/carbon-to-nitrogen ratios/medium optimization/microbial biomass分类
生物学引用本文复制引用
王晨,卢雅欣,李晓勇,张嘉祎,许乐天,冯庆..碳氮元素数量比对典型耐盐微生物增殖影响[J].山东科学,2025,38(2):73-79,7.基金项目
齐鲁工业大学(山东省科学院)国际合作研究专项资金计划项目(QLUTGJHZ2018005) （山东省科学院）
