水产学报2025，Vol.49Issue(5)：144-157,14.DOI:10.11964/jfc.20240514549
牛磺酸对大口黑鲈生长、糖脂代谢和抗氧化能力的影响
Effects of taurine on growth,glucolipids metabolism and hepatic antioxidant of largemouth bass(Micropterus salmoides)
摘要
Abstract
To evaluate the effects of taurine on the growth performance,hepatic glucolipids metabolism,and hepatic antioxid-ant capacities of largemouth bass(Micropterus salmoides),with an initial weight of(19.00±0.20)g were reared in cages(120 cm × 80 cm × 150 cm)for 8 weeks.The high starch control group(CON)was set at an 18％tapioca starch level,and 0.05％and 0.10％taurine(T0.05 and T0.1)were added based on this control group.The experiment consisted of three treatments,each with three replicates of 50 fish per replicate.Results indicated that taurine addition to high-starch diets significantly increased weight gain rate(WGR)and specific growth rate(SGR),while significantly decreasing liver crude fat and hepatic glycogen content.Intestinal amylase activity was also significantly elevated.Histological staining with H.E,PAS,and oil red O revealed that hepatocytes in the 0.1％taurine group were structurally intact,with reduced glycogen and fat droplets compared to the CON group.Serum glucose,low-density lipoprotein cholesterol,glutamic oxalacetic transaminase(GOT),and glutamate pyr-uvic transaminase(GPT)levels were significantly decreased after taurine addition,whereas alkaline phosphatase(ALP)and high-density lipoprotein cholesterol(HDL-C)levels were significantly increased.Hepatic G6Pase activity was significantly decreased in the taurine groups,while GK and PK activities were significantly elevated.The expression of genes related to gly-colysis(pfk,gk and glut2)was up-regulated,while the expression of hepatic lipid synthesis-related genes(dgat1,lxr-1,srebp1,acc1α,fasn and gpat4)was down-regulated.In contrast,the expression of lipolysis-related genes(hsl,mgl,pparα,lpl,atgl and acadm)was up-regulated.Compared to the CON group,the expression of antioxidant-related genes(cat,sod,il-10 and tgf-β)was up-regulated in the taurine gropus,while nf-Kb and il-8 expression was down-regulated.In summary,adding taurine to high-starch feeds can improve growth,regulate hepatic glucose-lipid metabolism,increase carbohydrate utilization,reduce hepatic glycogen deposition and lipid accumulation,and enhance the antioxidant capacity of M.salmoides.Under the experimental conditions,the recommended taurine addition in M.salmoides feed was 0.10％.This study provides a theoretical basis for taur-ine's role in regulating glucose and lipid metabolism and offers technical support for improving carbohydrate utilization in aquatic animals.关键词
大口黑鲈/牛磺酸/高淀粉/糖脂代谢/抗氧化Key words
Micropterus salmoides/taurine/high-starch/glucolipid metabolism/antioxidant分类
农业科技引用本文复制引用
边延欣,谢凯,蔡明浪,朱波,周锡勋,胡毅,张俊智..牛磺酸对大口黑鲈生长、糖脂代谢和抗氧化能力的影响[J].水产学报,2025,49(5):144-157,14.基金项目
国家自然科学基金(2019YFD0900200) National Nature Science Foundation of China(2019YFD0900200) （2019YFD0900200）
