山东医药2025，Vol.65Issue(4)：21-25,5.DOI:10.3969/j.issn.1002-266X.2025.04.005
载血人参石油醚部位胶束-白及多糖水凝胶对大鼠慢性创面愈合的促进作用
Promoting effect of blood-loaded petroleum ether extract of Indigofera stachyodes micelles-Bletilla striata polysaccharide hydrogel on chronic wound healing in rats
摘要
Abstract
Objective To observe the promoting of blood-loaded petroleum ether extract of Indigofera stachyodes mi-celles-Bletilla striata polysaccharide hydrogel(ISs-M-Gel)on chronic wound healing in rats.Methods Eighty male SPF SD rats were randomly divided into groups A,B,C,D,and E,with 16 rats in each group.A 2.5 cm×2.5 cm wound was made on the back of rats in each group.The chronic wound model was prepared by intramuscular injection of penicillin po-tassium(4,000 units/day,once a day,for four consecutive days)and hydrocortisone acetate(80 mg/kg,once a day,for seven consecutive days).After the chronic wound models of rats were successfully constructed,the chronic wound in the group A was applied with pure water,the chronic wound in the group B was applied with bovine basic fibroblast growth fac-tor external solution,the chronic wound in the group C was applied with the petroleum ether extract of Indigofera stachyo-des(ISs),the chronic wound in the group D was applied with blank micelle-Bletilla striata polysaccharide hydrogel(Blank-M-Gel),and the chronic wound in the group E was applied with ISs-M-Gel.The wounds on the 3rd,7th,14th,and 21st days of treatment were photographed,and the wound healing rate was calculated.At the above time point,the blood of rats in each group was collected to prepare serum,serum interleukin-6(IL-6)and tumor necrosis factor α(TNF-α)were detected by enzyme-linked immunosorbent assay,and wound regenerated skin tissues were obtained for HE and Mas-son staining.Total superoxide dismutase(SOD)and malondialdehyde(MDA)kits were used to detect SOD and MDA con-tent in wound-regenerated skin tissues.Immunohistochemical method was used to detect platelet/endothelial cell adhesion molecule-1(CD31)in wound-regenerated skin tissues to evaluate angiogenesis.Results On the 14th and 21st days of intervention,compared with the group A,the wound healing rate in the other groups increased(P<0.05).On the 7th and 14th days of intervention,compared with the group A,the serum levels of IL-6 and TNF-α in the groups B and E de-creased(all P<0.05);the SOD increased and MDA decreased in the wound-regenerated skin tissues in the group B and group E on the 14th and 21st days of intervention.Compared with other administration groups,the regenerated skin fiber tissue deposition was dense in the group B and group E.On the 14th and 21st days of the intervention,compared with the group A,the CD31 positivity of wound-regenerated skin tissues of rats in the groups B,C,and E increased,and the angio-genesis was the best.Conclusion ISs-M-Gel can promote chronic wound healing in rats,and its effect is better than those of ISs and Blank M-Gelm and is similar to that of bovine basic fibroblast growth factor external solution.关键词
载血人参石油醚部位胶束-白及多糖水凝胶/牛碱性成纤维细胞生长因子外用溶液/慢性创面/创面愈合Key words
blood-loaded petroleum ether extract of Indigofera stachyode micelles-Bletilla striata polysaccharide hy-drogel/bovine basic fibroblast growth factor external solution/chronic wound/wound healing分类
医药卫生引用本文复制引用
郑力凡,宋选飞,钟靓,肖思羽,赵婵,刘耀,徐剑,张永萍..载血人参石油醚部位胶束-白及多糖水凝胶对大鼠慢性创面愈合的促进作用[J].山东医药,2025,65(4):21-25,5.基金项目
贵州省科技计划项目[黔科合中引地(2023)006] （2023）
贵州省高层次创新型人才项目[黔科合平台人才-GCC(2023)037] （2023）
贵州省教育厅滚动支持省属高校科研平台团队项目[黔教技(2022)022]. （2022）
