水生生物学报2025，Vol.49Issue(5)：73-85,13.DOI:10.7541/2025.2024.0407
利用鱼类性别控制技术创制YY超雄和XY全雄乌苏里拟鲿
PRODUCTION OF YY SUPER-MALE AND XY ALL-MALE PSEUDOBAGRUS USSURIENSIS BY SEX CONTROL TECHNOLOGY
摘要
Abstract
In this study,we investigated the effects of various doses and treatment durations of 17β-estradiol(E2)on the growth,survival rate,and sex ratio of P.ussuriensis.From 10 to 70dph(days post-hatching),feeding P.ussuriensis diets containing 10,50,and 100 mg/kg E2 resulted in a 100％feminization rate.However,the growth and survival rates decreased with increasing E2 concentration,and all fish in the group fed with 150 mg/kg E2 died.Overall,10 mg/kg E2 treatment from 10 to 40dph was found to be the optimal approach for the feminization of P.ussuriensis.The expres-sion levels of male key sex differentiation-related genes,such as dmrt1,amh,and cyp17a1,were significantly lower in XY females than that of XY males.The expression levels of female key sex differentiation-related genes including cyp19a1a,zar1,and gdf9 in XY females were consistent with those in XX females and were significantly higher than that in XY males.Additionally,whole-genome resequencing revealed 3777645 single nucleotide polymorphisms(SNPs)and 1287509 insertions/deletions(Indels)in 15 female and 15 male P.ussuriensis.Among these,99601 were identified as sex-linked SNPs and 27614 as sex-linked Indels.The sex-linked SNPs were enriched in the 6.84 and 23.82 Mb regions of chromosome 8.X and Y chromosome specific molecular marker were developed based on sex-linked Indels,which proved effective for the sex identification in P.ussuriensis.These markers were well applied to wild populations from the Heilongjiang River as well as farmed populations in Henan and Hubei Province.YY super-male were identi-fied from the offsprings of XY male mating with XY female.Subsequently,using YY super-male P.ussuriensis as the male parent,an all-male population was bred with a 100％male ratio.The all-male population at one-year old was 1.54 times heavier than the mixed-sex population.In summary,10 mg/kg E2 treatment from 10 to 40dph is the optimal approach for the feminization of P.ussuriensis.The sex-linked molecular markers developed by whole-genome rese-quencing can effectively identify the genotype of P.ussuriensis and help produce YY super-male fish.XY all-male P.ussuriensis had the characteristics of uniform size and fast growth performance which will be more popular among farmers.关键词
性别连锁分子标记/17β-雌二醇/性逆转/乌苏里拟鲿Key words
Sex-specific molecular markers/17β-estradiol/Sex reversal/Pseudobagrus ussuriensis分类
水产学引用本文复制引用
蒋有渤,孙瑞东,谢焱,程宜,陈见,李佩,王忠卫,熊阳,梅洁..利用鱼类性别控制技术创制YY超雄和XY全雄乌苏里拟鲿[J].水生生物学报,2025,49(5):73-85,13.基金项目
湖北省杰青项目——长江特色鱼类精准性控育种的遗传学基础(2021CFA057)资助[Supported by Excellent Youth Founda-tion of Hubei Provincial Natural Science Foundation(2021CFA057)] （2021CFA057）
