水生生物学报2025，Vol.49Issue(5)：86-94,9.DOI:10.7541/2025.2024.0454
长江江豚和东亚江豚染色体水平基因组微卫星分布特征分析
MICROSATELLITES DISTRIBUTION CHARACTERIZATION IN THE CHRO-MOSOME-LEVEL GENOMES OF THE YANGTZE FINLESS PORPOISE AND THE EAST ASIAN FINLESS PORPOISE
摘要
Abstract
In this study,bioinformatic methods were used to analyze and compare the genome-wide characterization of microsatellites in the chromosome-level genomes(2.62 and 2.50 Gb respectively)of the Yangtze finless porpoise(Neophocaena asiaeorientalis asiaeorientalis;YFP)and the East Asian finless porpoise(N.a.sunameri;EAFP).The results showed that a total of 888424 and 867792 perfect microsatellites were identified in the whole genomes of the YFP and the EAFP,with a total length of 1534077 and 1472448 bp respectively,accounting for about 0.59％of the whole genome sequences.In both the YFP and EAFP genomes,the numbers of the six major repeat types of perfect microsatellites were similar,with mononucleotide repeats being the most abundant,followed by dinucleotide,hexanu-cleotide,tetranucleotide,trinucleotide,and pentanucleotide repeats.The most abundant repeat motifs in the two genomes were A,AC,AT,CCCTAA,and AGGGTT.Additionally,we also analyzed the microsatellite distribution characteristics in the Y chromosome of the YFP and EAFP.In total,6195 and 5024 perfect microsatellites were detected in the Y chromosomes respectively.Among the six repeat types of perfect microsatellites on the Y chromo-some,mononucleotide repeat was also the most abundant type,followed by dinucleotide,tetranucleotide,trinucleotide,pentanucleotide,and hexanucleotide.In addition,A,AC,and AAT repeat motif categories were the most dominant.In this study,we examined the distribution characteristics and disparities of microsatellites in the chromosomal-level genomes of the YFP and the EAFP,thereby establishing a solid foundation for future research on the development and utilization of species specific microsatellite markers.关键词
长江江豚/东亚江豚/基因组/Y染色体/微卫星Key words
the Yangtze finless porpoise/the East Asian finless porpoise/Genome/Y chromosome/Microsatellite分类
生物科学引用本文复制引用
李寒姝,杨连东,孟明辉,许攀,范飞,王丁,郑劲松..长江江豚和东亚江豚染色体水平基因组微卫星分布特征分析[J].水生生物学报,2025,49(5):86-94,9.基金项目
国家重点研发计划(2022YFF1301600) （2022YFF1301600）
国家自然科学基金(31870372) （31870372）
中国科学院科技服务网络计划(KFJ-STS-QYZD-2021-27-001)资助[Supported by National Key Programme of Research and Development(2022YFF1301600) （KFJ-STS-QYZD-2021-27-001）
the National Natural Science Foundation of China(31870372) （31870372）
Science and Technology Service Network Initiative,Chinese Academic of Sciences(KFJ-STS-QYZD-2021-27-001)] （KFJ-STS-QYZD-2021-27-001）
