水生生物学报2025，Vol.49Issue(5)：95-101,7.DOI:10.7541/2025.2024.0343
静水压诱导三倍体勃氏雅罗鱼的研究
HYDROSTATIC INDUCTION OF TRIPLOID LEUCISCUS BRANDTI(DYBOWSKI)
摘要
Abstract
In this study,the optimum conditions for hydrostatic induction of triploid Leuciscus brandti were selected by means of histological and comparative tests.The results showed that:(1)The tissue section examined 5min after fertili-zation showed that the second polar body was obviously protruding from the egg membrane of the fertilized egg,indi-cating its readiness for discharge.(2)The fertilization rate of 5min initiation time was not significantly different from the control group(P＞0.05),but was significantly higher than that of other experimental groups(P＜0.05).There was no significant difference in incubation rate between 4min and 5min(P＞0.05),but it was significantly lower than that of the control group(P＜0.05),and significantly higher than that of other experimental groups(P＜0.05).The triploid rate of the initial time of 5min was significantly higher than that of other experimental groups(P＜0.05).(3)There was no significant difference between the fertilization rate of hydrostatic pressure 60 MPa and control group(P＞0.05),but it was significantly higher than that of other experimental groups(P＜0.05).The hatching rate of 65 MPa was signifi-cantly lower than that of other groups(P＜0.05),and the hatching rate of 50,55 and 60 MPa had no significant diffe-rence(P＞0.05),but was significantly lower than that of the control group(P＜0.05).The triploid rate of 60 MPa was significantly higher than that of other experimental groups(P＜0.05).(4)There was no significant difference in fertiliza-tion rate between 4 and 5min induction duration(P＞0.05),but it was significantly higher than that of other experimen-tal groups(P＜0.05),and significantly lower than that of the control group(P＜0.05).The hatching rate of experimental groups was significantly lower than that of the control group(P＜0.05),and the hatching rate and triploid rate lasting 4min were significantly higher than those of other experimental groups(P＜0.05).Under the conditions of this experi-ment,with a water temperature of(17±1)℃,the best induction conditions for triploid Leuciscus brandti were 5min after fertilization,the hydrostatic pressure of 60 MPa,and the continuous treatment duration of 4min.关键词
静水压/三倍体/倍性鉴定/诱导条件/勃氏雅罗鱼Key words
Hydrostatic pressure/Triploidy/Ploidy identification/Induction conditions/Leuciscus brandti分类
水产学引用本文复制引用
于涵,郭贵良,牛小天,王桂芹,陈秀梅,邵云龙,宋健,孔祎頔,李民..静水压诱导三倍体勃氏雅罗鱼的研究[J].水生生物学报,2025,49(5):95-101,7.基金项目
吉林省科技发展计划(20240303067NC) （20240303067NC）
国家特色淡水鱼产业技术体系(CARS-46)资助[Supported by the Jilin Province Science and Technology Development Plan Project(20240303067NC) （CARS-46）
National Characteristic Freshwater Fish Industry Techno-logy System(CARS-46)] （CARS-46）
