水生生物学报2025，Vol.49Issue(5)：102-120,19.DOI:10.7541/2025.2024.0450
龟鳖目表皮分化复合体基因的比较基因组学鉴定与分析
COMPARATIVE GENOMIC IDENTIFICATION AND ANALYSES OF EPIDER-MAL DIFFERENTIATION COMPLEX GENES IN TURTLES
摘要
Abstract
The epidermal differentiation complex(EDC)is a cluster of genes highly associated with keratinocytes differentiation and epidermal keratinization.This gene cluster plays an important role in the differentiation of epider-mal tissues in amniotes,the formation of various skin appendages,and the keratinization process.Therefore,exploring the sequence characteristics and evolution of EDC genes in turtles can help elucidate the genetic basis and evolutionary history of their shell epidermis traits.Currently,there is a lack of systematic comparative analysis of the sequence cha-racteristics and evolutionary levels of EDC genes across different turtle groups.This study performed comparative genomics identification of EDC in 28 turtles,including chromosomal localization,amino acid composition,domain analysis,protein spatial structure analysis,and the reconstruction of evolutionary history.The EDC genes in turtles exhibit the same four gene families found in other reptiles,with members of the SEDC gene family expanding through multiple tandem repeat events.The amino acid composition of turtle SEDC proteins is highly diverse,with SEDC and SFTP protein amino acid content showing group-specific characteristics(hard-shelled,soft-shelled,leatherback),which supports the diversity of shell epidermal structures in turtles.The functional domains and protein spatial structures of S100A and SFTP are highly conserved across turtle species,with motif 3 of PGLYRP3 showing a loss specific to the family Trionychidae.The turtle-specific EDPCV originates from EDP1,and mutations have enriched cysteine and valine,facilitating gene expansion through tandem duplication.This study provides a data foundation and theoretical basis for further research on the role of EDPCV in turtles.关键词
表皮分化复合体/氨基酸组成/基因扩张/EDPCV基因/龟鳖Key words
EDC/Amino acid content/Gene expansion/EDPCV gene/Turtles分类
生物学引用本文复制引用
陈孟饶,李芳,陈其然,黄保友,孙伟,钱国英,葛楚天,王宗吉..龟鳖目表皮分化复合体基因的比较基因组学鉴定与分析[J].水生生物学报,2025,49(5):102-120,19.基金项目
国家自然科学基金(32102772) （32102772）
宁波市自然科学基金(2023J043) （2023J043）
浙江省高校基础科研经费项目 （）
2022年浙江省"生物工程"一流学科(ZS2022008)资助[Supported by the Natural Science Foundation of China(32102772) （ZS2022008）
the Ningbo Natural Science Foun-dation(2023J043) （2023J043）
the Basic Scientific Research Foundation of Zhejiang Provincial Universities （）
the Zhejiang Provincial Top Discipline of Biological Engineering(Level A),Zhejiang Wanli University(ZS2022008)] （Level A）
