色谱2025，Vol.43Issue(5)：399-412,14.DOI:10.3724/SP.J.1123.2024.11001
单细胞与单颗粒外泌体分离分析进展与展望
Progress and prospect of separation and analysis of single-cell and single-particle exosomes
摘要
Abstract
Exosomes are nanoscale vesicles secreted by cells and are encapsulated in lipid bilayers.They play crucial roles in cell communication and are involved in a variety of physio-logical and pathological processes,including immune regulation,angiogenesis,and tumor initi-ation and metastasis.Exosomes carry a variety of biomolecules from maternal cells and are therefore important vehicles for discovering disease markers.Traditional detection methods only provide average cell-population information for a given sample and cannot establish clear relationships between the biological functions of exosomes and subtype owing to the significant heterogeneity associated with exosomes from different cell subsets.Therefore,characterizing exosomes at the single-cell and single-particle levels requires exosome specificities to be further explored and the characteristics of various exosome subtypes to be distinguished.Commonly used single-particle exosome characterization technologies include flow cytometry,super-resolution microscopy,atomic force microscopy,surface-enhanced Raman spectroscopy,prox-imity barcoding assay and MS.In this paper,we summarize recent advances in the separation and characterization of single-cell exosomes based on microfluidics and provide future applica-tions prospects for emerging technologies(such as Olink proteomics,click chemistry,and molecular imprinting)for studying single-cell and single-particle exosomes.关键词
单细胞外泌体/单颗粒外泌体/分离与分析/微流控技术/综述Key words
single-cell exosomes/single-particle exosomes/separation and analysis/microflu-idics/review分类
化学化工引用本文复制引用
卜爱香,武光耀,胡良海..单细胞与单颗粒外泌体分离分析进展与展望[J].色谱,2025,43(5):399-412,14.基金项目
国家自然科学基金(22374056).National Natural Science Foundation of China(No.22374056). （22374056）
