色谱2025，Vol.43Issue(5)：413-423,11.DOI:10.3724/SP.J.1123.2024.11004
基于亲和作用力的外泌体高效分离方法的经典策略和研究进展
Typical strategy and research progress of efficient isolation methods of exosomes based on affinity interaction
摘要
Abstract
Exosomes form a subclass of extracellular vesicle that are secreted by most cells and found in nearly all body fluids,including blood,urine,saliva,amniotic fluid,and milk,as well as in various tissues and intercellular spaces.Exosomes have recently been recognized as cruci-al intercellular communication mediators,and an increasing number of studies have shown that exosomes are important liquid-biopsy tools that play irreplaceable roles in the diagnosis,prog-nosis,and treatment of diseases.The ability to isolate high-quality exosomes is a prerequisite for diagnosing and subsequently treating diseases in an accurate and repeatable manner.Howev-er,efficiently isolating exosomes from complex biological samples is challenging owing to their relatively low abundances and interference from non-vesicular macromolecules(such as cell debris and proteins).To date,various isolation techniques based on the physical,chemical,and biological characteristics of exosomes have been developed.Indeed,efficient affinity-inter-action-based methods have recently overcome the limitations and drawbacks of traditional exo-some isolation methods and are widely used in scientific research and clinical applications.This review focuses on exosome isolation and enrichment,and systematically reviews recent re-search progress on efficient isolation methods based on affinity interactions.Developmental prospects of exosome isolation and enrichment directions are analyzed with the aim of provi-ding a reference for the construction and use of new exosome-isolation strategies.关键词
外泌体/生物标志物/亲和作用/分离富集/研究进展Key words
exosomes/biomarkers/affinity interaction/isolation and enrichment/research progress分类
化学化工引用本文复制引用
王海燕,谢沛涓,乔晓强,张丽媛..基于亲和作用力的外泌体高效分离方法的经典策略和研究进展[J].色谱,2025,43(5):413-423,11.基金项目
国家自然科学基金(22274035,21974017) （22274035,21974017）
河北大学创新团队(IT2023A06).National Natural Science Foundation of China(Nos.22274035,21974017) （IT2023A06）
Innovation Team Program of Hebei University(No.IT2023A06). （No.IT2023A06）
