水生生物学报2025，Vol.49Issue(5)：185-196,12.DOI:10.7541/2025.2024.0399
厚壳贻贝外套膜对细菌肽聚糖胁迫的代谢响应
METABOLIC RESPONSE OF MYTILUS CORUSCUS MANTLE TO PEPTIDOGLYCAN STRESS
摘要
Abstract
Mytilus coruscus is a shellfish with huge economic importance in China,and the mantle is an important immune-related organ.To investigate the immune function of the mantle and its underlying mechanisms,peptidogly-can(PGN)was used as a stressor,and ultra-high-pressure liquid chromatography-mass spectrometry technique was employed to identify the composition and content of metabolites from the mantle at 48h of post peptidoglycan stress.In addition,free amino acids,antimicrobial activities,and antioxidant activities of the mantle were analyzed and compared between the control and stressed samples.The results revealed that PGN stress induced significant changes in mantle metabolites,and a total of 486 metabolites with significant difference(SDMs)was identified,including 232 up-regu-lated and 254 down-regulated SDMs(P＜0.05).Among these,lipids and lipid-like molecules,organic acids and their derivatives,and organo-heterocyclic compounds were the most prominent SDMs.KEGG enrichment analysis further revealed that these SDMs were enriched in different pathways,showing a complex response of the mantle to the PGN stress.Notably,the up-regulated SDMs were enriched in pathways related to cell signaling and amino acid metabolism,while down-regulated SDMs were enriched in lipid metabolisms,vitamin related metabolisms,and autophagy.Gene set enrichment analysis(GSEA)further confirmed the regulation of some SDMs and the enrichment of aminoacyl-tRNA biosynthesis and autophagy-animal.In addition,free amino acid analysis also confirmed the up-regulation(P＜0.05)of certain amino acids,such as aspartic acid,glutamic acid,and arginine,in the mantle under PGN stress.Enzymatic acti-vity analysis revealed an increase(P＜0.05)of catalase activity and a decrease of hydrogen peroxide in the PGN stressed mussel mantle.Interestingly,antimicrobial function analysis revealed that the mucus from PGN stressed mantle presented stronger inhibition against Bacillus subtilis,indicating that PGN stress induce stronger antibacterial activity in mussel mantle.These findings indicated that the mussel mantle may respond to PGN stress by enhancing immune capacity,regulating inflammatory balance,and inhibiting cell autophagy.This study provides valuable insights into the molecular strategies of mussel mantle in response to immune stress and offers a scientific basis for future efforts to promote healthy aquaculture of mussels.关键词
外套膜/肽聚糖/免疫/代谢组学/厚壳贻贝Key words
Mantle/Peptidoglycan/Immunity/Metabolomics/Mytilus coruscus分类
生物科学引用本文复制引用
杨子霖,范孝俊,肖文慧,曹萍麟,张晓林,严小军,廖智..厚壳贻贝外套膜对细菌肽聚糖胁迫的代谢响应[J].水生生物学报,2025,49(5):185-196,12.基金项目
国家自然科学基金面上项目(32271580) （32271580）
国家自然科学基金重点国际合作与交流项目(42020104009)资助[Supported by the National Natural Science Foundation of China(32271580 and 42020104009)] （42020104009）
