水生生物学报2025，Vol.49Issue(5)：197-208,12.DOI:10.7541/2025.2024.0316
红毛菜应答失水和复水的生理表现和转录组学分析
PHYSIOLOGICAL PERFORMANCE AND TRANSCRIPTIONAL ANALYSIS OF BANGIA FUSCOPURPUREA IN RESPONSE TO DEHYDRATION AND REHYDRATION
摘要
Abstract
Thalli of the economically important red alga Bangia fuscopurpurea mainly inhabit the intertidal rocks.Consequently,they experience approximately two dehydration/rehydration cycles per day.During the tidal cycle,the thalli can lose around 90％of water at low tide and rapidly recover to normal water content after rehydration,indicat-ing that B.fuscopurpurea has the ability to adapt to the rapid water changes induced by the tidal cycle.However,litter is known about the mechanisms by which B.fuscopurpurea adapts to tidal changes.Therefore,a wild strain of B.fuscopurpurea was used to assess its response to 2h dehydration(with 5 water loss rates:0,30％,60％,90％)and 2h rehydration(pretreated with 2h dehydration at a 90％water loss rate)by examining physiological traits and the tran-scriptome.The present study found that the maximum photochemical quantum yield(Fv/Fm)of B.fuscopurpurea decreased significantly with the increasing dehydration level.Meanwhile,the contents of superoxide anion(O2)and hydrogen peroxide(H2O2)increased significantly and peaked at 30％and 60％water loss,respectively.The content of malondialdehyde(MDA)also increased with dehydration level.After rehydration,Fv/Fm and contents of O2-H2O2,and MDA returned to the levels of 0 water loss rate(WL0％)treatment group.Activities of superoxide dismutase(SOD)showed a decreasing trend with increasing desiccation.Ascorbate peroxidase(APX)and peroxidase(POD)increased significantly by dehydration,both peaking at 60％water loss,while catalase(CAT)did not change significantly.After rehydration,the activities of SOD,CAT,APX,and POD all returned to the levels of the WL 0％treatment.Dehydra-tion significantly elevated proline and soluble sugar contents of B.fuscopurpurea.Additionally,the contents of phycoe-rythrin and allophycocyanin increased markedly at 60％water loss.These findings suggest that the algae's adaptation to dehydration is facilitated by enhanced intracellular antioxidant enzyme activities and antioxidant contents,and by reducing photosynthesis to minimize cell damage.In addition,by comparing the transcriptional expression profiles of B.fuscopurpurea under different dehydration and rehydration treatmetns,we screened 3043 differential genes.Diffe-rential gene enrichment analysis revealed that genes related to ribosomal proteins and heat-excited proteins were up-regulated by dehydration treatment,while genes related to amino acid synthesis,aminoacyl-tRNA synthetase(aaRS),and ribosomal biogenesis-mediated ribosomal protein-related genes were significantly down-regulated by rehydration.This study further analyzed the expression patterns of these key genes and found that algae maintain intracellular homeo-stasis by synthesizing dehydration-tolerant ribosomal proteins and initiating defense functions such as molecular chape-rones.In response to rehydration,the inhibition of amino acid metabolism,aaRS,and ribosome biogenesis-mediated synthesis of ribosomal proteins was employed to conserve energy.In addition,efforts were made to enhance the energy supply from the light reactions of photosynthesis and the transport of toxic metabolites to assist B.fuscopurpurea in resuming metabolic activities.In summary,this study analyzed the physiological and transcriptional responses of B.fuscopurpurea to dehydration and rehydration,and identified key response genes.The results can contribute to a deeper understanding of the response of intertidal macroalgae to rapid water changes,as well as provide a data reference for the selection and breeding of B.fuscopurpurea.关键词
红毛菜/失水/复水/生理响应/抗氧化系统/转录组/差异表达基因Key words
Bangia fuscopurpurea/Dehydration/Rehydration/Physiological response/Antioxidant system/Transcrip-tome/Differentially expressed gene分类
农业科技引用本文复制引用
张亚文,林密,许凯,徐燕,纪德华,王文磊,陈昌生,谢潮添..红毛菜应答失水和复水的生理表现和转录组学分析[J].水生生物学报,2025,49(5):197-208,12.基金项目
福建省自然科学基金(2022J01801) （2022J01801）
农业农村部岗位科学家(GARS-50-2021) （GARS-50-2021）
国家自然科学基金(42376109和U21A20265) （42376109和U21A20265）
国家重点研发计划重点专项课题(2023YFD2400103) （2023YFD2400103）
福建省科技计划项目区域发展项目(2023N3002)资助[Supported by the Natural Science Foundation of Fujian Province(2022J01801) （2023N3002）
China Agriculture Research System(CARS-50-2021) （CARS-50-2021）
National Natural Science Foundation of China(42376109 and U21A20265) （42376109 and U21A20265）
National Key Research and Development Plan for Key and Special Projects(2023YFD2400103) （2023YFD2400103）
Regional Development Project of Fujian Science and Technology Program(2023N3002)] （2023N3002）
