中国海洋大学学报（自然科学版）2025，Vol.55Issue(5)：70-86,17.DOI:10.16441/j.cnki.hdxb.20230160
去甲基甲基萘醌菌LLJ775的全基因组测序和比较基因组学分析
Genome Sequencing and Comparative Genomics Analysis of Demequina sp.LLJ775
摘要
Abstract
To explore the metabolic mechanisms and secondary metabolite synthesis capacity of a novel strain Demequina sp.LLJ775 isolated from the hydrothermal vent of the Okinawa Trough in extreme marine environments,this study conducted a comparative genomics analysis of this strain and related reference strains.The genome of Demequina sp.LLJ775 was sequenced using whole genome sequen-cing.The evolutionary status of this strain was determined through phylogenetic analyses.Functional annotation and comparative genomics analyses of Demequina sp.LLJ775 and reference strains were per-formed to explore the metabolic characteristics and genetic potential of strains within this genus.The genome size of Demequina sp.LLJ775 was 2,822,402 bp,with the GC content of 69.3％and 2,738 protein-coding genes.Phylogenetic analyses of 16S rRNA genes and single-copy core genes revealed that this strain had low sequence similarities to its closely related strains,with the highest 16S rRNA gene sequence similarity being 98.41％.This preliminary result suggested that it was a novel species within the genus Demequina.Functional annotation analysis revealed that the proportion of genes related to replication,recombination,and repair in Demequina sp.LLJ775 was higher than in the reference strains.The presence of genes involved in metabolic pathways such as the glyoxylate shunt,nitrite re-duction and encoding for ferric iron ABC-type transporter protein enhanced the adaptability of Demequi-na sp.LLJ775 to the extreme hydrothermal environment.Additionally,carbohydrate-active enzymes such as carbonic anhydrase,β-glucosidase,isoamylase and hyaluronan synthase were annotated in De-mequina sp.LLJ775.Moreover,gene clusters related to lycopene synthesis and other secondary metab-olite synthesis pathways were identified in Demequina sp.LLJ775,suggesting that this strain had sig-nificant application potential.The metabolic characteristics of the potential novel species Demequina sp.LLJ775 were revealed through whole genome sequencing and comparative genomics analysis.This study provided a reference for further understanding of the adaptation mechanisms of Demequina sp.LLJ775 to hydrothermal environments and offered a theoretical foundation for exploring its potential applica-tion.关键词
冲绳海槽/深海热液区/去甲基甲基萘醌菌LLJ775/全基因组测序/比较基因组学分析Key words
Okinawa Trough/deep sea hydrothermal vents/Demequina sp.LLJ775/whole genome sequencing/comparative genomics analysis分类
生物科学引用本文复制引用
孙凯,尹福,刘莉君,王双,张晓华,于敏..去甲基甲基萘醌菌LLJ775的全基因组测序和比较基因组学分析[J].中国海洋大学学报（自然科学版）,2025,55(5):70-86,17.基金项目
国家自然科学基金项目(41976137,92251303,U1706208)资助Supported by the National Natural Science Foundation of China(41976137,92251303,U1706208) （41976137,92251303,U1706208）
