时珍国医国药2025，Vol.36Issue(7)：1218-1224,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-0704
糖毒清干预AHR/IL-22信号通路改善2型糖尿病胰岛素抵抗机制的研究
Study on the mechanism of Tangduqing(糖毒清)in ameliorating insulin resistance of type 2 diabetes mellitus via intervening the AHR/IL-22 signaling pathway
摘要
Abstract
Objective To investigate the mechanism of Tangduqing(糖毒清,TDQ)in ameliorating insulin resistance in type 2 diabetic rats through the aryl hydrocarbon receptor(AHR)/interleukin-22(IL-22)signaling pathway.Methods Sixty SDrats were divided into the normal control group(n=10)and model group(n=50).Type 2 diabetic models were established with 50 rats by intraperitone-al injection of streptozotocin(STZ).The 44 successfully modeled rats were randomly and equally divided into the model group,metform-in hydrochloride group,TDQ low-dose group,and TDQ high-dose group.After six weeks of continuous gavage,the area under the curve(AUC)was tested using the oral glucose tolerance test(OGTT),insulin resistance was evaluated using homeostasis model assess-ment of insulin resistance(HOMA-IR)and quantitative insulin sensitivity index(ISI).Indole contents were measured via liquid chro-matograph mass spectrometer(LC-MS).Serum levels of IL-22 and IL-6 were detected by ELISA.Immunohistochemistry and HE staining were used to assess the intestinal tissues and the expressions of AHR and IL-22.Results TDQ reduced blood glucose,fasting insulin(FINS),AUC,and HOMA-IR(P<0.05)while increased ISI(P<0.05)in STZ-induced diabetic rats.It elevated bacteri-al metabolite indole levels,suppressed IL-6 level in serum(P<0.05),enhanced IL-22(P<0.05),alleviated intestinal inflamma-tory infiltration,and up-regulated AHR and IL-22 expression in the intestinal tissues.Conclusion TDQ ameliorates insulin resistance and suppresses metabolic inflammation,and its mechanism may be associated with the activation of the AHR/IL-22 inflammatory path-way.关键词
AHR/IL-22信号通路/代谢炎症/胰岛素抵抗/糖毒清Key words
AHR/IL-22 signaling pathway/Metabolic inflammation/Insulin resistance/Tangduqing(糖毒清)分类
医药卫生引用本文复制引用
赵朱佳,李光美,赵伟,陈莉,吴青,李珊珊,黄紫若,熊丽娟..糖毒清干预AHR/IL-22信号通路改善2型糖尿病胰岛素抵抗机制的研究[J].时珍国医国药,2025,36(7):1218-1224,7.基金项目
国家自然科学基金地区基金(82260875) （82260875）
贵州中医药大学第二附属医院院内科研项目(GZEYK[2020]8号) （GZEYK[2020]8号）
