时珍国医国药2025，Vol.36Issue(7)：1225-1233,9.DOI:10.70976/j.1008-0805.SZGYGY-2025-0705
基于HIF-1α/VEGF信号通路探讨敦煌疗风虚瘦弱方拮抗PCOS卵巢纤维化的作用机制
Exploring the mechanism of Dunhuangliao Fengxu Shouruo Formula(敦煌疗风虚瘦弱方)in antagonizing ovarian fibrosis of PCOS rats based on the HIF-1α/VEGF signaling pathway
摘要
Abstract
Objective To investigate the therapeutic effect of the Dunhuangliao Fengxu Shouruo Formula(敦煌疗风虚瘦弱方,DFSF)on ovarian fibrosis in the letrozole-induced polycystic ovary syndrome(PCOS)model rats,based on the hypoxia-inducible factor-1α/vascular endothelial growth factor(HIF-1α/VEGF)pathway.Methods Forty-eight female SD rats were randomly divided into different groups.A PCOS ovarian fibrosis model was established using letrozole.The Yousiyue group(优思悦组)received 0.01 g·kg-1·d-1 of drospirenone and ethinylestradiol tablets via gavage,while the low/medium/high-dose DFSF groups received 3.10,6.21,and 12.42 g·kg-1 of DFSF respectively,for 28 consecutive days.Vaginal smears were used to dynamically monitor the estrous cycle.Hematoxylin-eosin(HE)staining was employed to observe ovarian histopathological changes.Serum levels of follicle-stimula-ting hormone(FSH),luteinizing hormone(LH),testosterone(T),and estradiol(E2)were measured using ELISA.RT-qPCR and immunohistochemistry(IHC)were used to assess mRNA and protein expression of the HIF-1α/VEGF pathway in the ovarian tissues.Western blot(WB)was performed to detect protein level of the HIF-1α/VEGF pathway and fibrosis-related markers of TGF-β1,α-SMA,and E-cad.Results Compared with the blank group,the model group showed increased body weight,elevated LH,T levels,and LH/FSH ratio(P<0.01),and reduced E2 level(P<0.05).Ovarian tissues exhibited up-regulated mRNA expressions of HIF-1α and VEGF(P<0.01),increased protein levels of HIF-1α,VEGF,TGF-β1,and α-SMA,and decreased E-cad expression(P<0.01).Both the Yousiyue group and all dose DFSF groups demonstrated reduced mRNA expressions of HIF-1α and VEGF(P<0.01,P<0.05),decreased protein levels of HIF-1α,VEGF,TGF-β1,and α-SMA,and increased E-cad(P<0.01,P<0.05),with the high-dose DFSF group showing the most significant effects.Conclusion DFSF ameliorates general conditions,sex hor-mone levels,and ovarian fibrosis in letrozole-induced PCOS rats.Its therapeutic mechanism may be associated with the regulation of the HIF-1α/VEGF signaling pathway.关键词
多囊卵巢综合征/HIF-1α/VEGF信号通路/敦煌疗风虚瘦弱方/卵巢纤维化Key words
Polycystic ovary syndrome/HIF-1α/VEGF signaling pathway/Dunhuangliao Fengxu Shouruo Formula(敦煌疗风虚瘦弱方)/Ovarian fibrosis分类
医药卫生引用本文复制引用
张小花,林祥羽,毛海燕,张志瑞,武权生,李鹤,芮守月,蔺文娟,黄灿灿,李善政,袁荣荣,万文文,张舒媛..基于HIF-1α/VEGF信号通路探讨敦煌疗风虚瘦弱方拮抗PCOS卵巢纤维化的作用机制[J].时珍国医国药,2025,36(7):1225-1233,9.基金项目
国家自然科学基金项目(82260949) （82260949）
甘肃省教育厅创新基金项目(2022A-065) （2022A-065）
甘肃省中医药管理局科研课题(GZKP-2023-44) （GZKP-2023-44）
