时珍国医国药2025，Vol.36Issue(7)：1234-1241,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-0706
基于TLR4/MyD88/NF-κB/NLRP3通路探讨膈下逐瘀汤加减方对慢性输卵管炎性不孕症的作用机制
Exploring the mechanism of modified Gexia Zhuyu Decoction(膈下逐瘀汤)in chronic salpin-gitis-induced infertility with qi stagnation and blood stasis syndrome based on the TLR4/MyD88/NF-κB/NLRP3 signaling pathway
摘要
Abstract
Objective To investigate the effects of modified Gexia Zhuyu Decoction(膈下逐瘀汤,GXZYD)on the the tissue structure of fallopian tubes in salpingitis-induced infertility(SOI)rats with qi stagnation and blood stasis based on the TLR4/MyD88/NF-κB/NLRP3 signaling pathway.Methods A disease-syndrome combined model of chronic SOI was established.After intervention with modi-fied GXZYT and the TLR4 pathway inhibitor TAK-242,plasma,serum,and fallopian tube tissues were collected.Serum levels of in-terleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were measured by enzyme-linked immunosorbent assay(ELISA).Hemorheological parameters were assessed to evaluate the state of qi stagnation and blood stasis.Pathological changes in fallopian tubes were observed by hematoxylin-eosin(HE)staining.Immunohistochemistry(IHC)was used to detect the expressions of TLR4 and NL-RP3.RT-Pcr and Western blot(WB)were performed to analyze mRNA and protein expression levels of TLR4,MyD88,NF-κB,NLRP3,Bax,and Bcl-2.Pregnancy rates were observed.Results Compared with the model group,the modified GXZYT group showed significantly reduced plasma viscosity andwhole bloodviscosity(3.0 s-1).Bothgroups exhibiteddecreasedserum IL-1βand TNF-α levels,down-regulated mRNA and protein expressions of TLR4,MyD88,NF-κB,NLRP3 and Bax,and up-regulated Bcl-2 mR-NA and protein expressions.Pathological status in fallopian tubes was alleviated,and pregnancy rates were increased in both groups.Conclusion Modified GXZYT alleviates chronic SOI with qi stagnation and blood stasis by modulating the TLR4/MyD88/NF-κB/NL-RP3 pathway,reducing apoptosis and inflammation.TAK-242 can play the role of modified GXZYT to improve inflammatory indexes and pathological state of the disease,but does not alleviate syndrome manifestations of traditional Chinese medicine(TCM).关键词
输卵管炎性不孕/气滞血瘀/TLR4/MyD88/NF-κB/NLRP3/凋亡/炎症Key words
Salpingitis-induced infertility/Qi stagnation and blood stasis/TLR4/MyD88/NF-κB/NLRP3/Apoptosis/In-flammation分类
医药卫生引用本文复制引用
董书含,杜云鹏,李楠,袁闻晗,汪海洋,刘丽..基于TLR4/MyD88/NF-κB/NLRP3通路探讨膈下逐瘀汤加减方对慢性输卵管炎性不孕症的作用机制[J].时珍国医国药,2025,36(7):1234-1241,8.基金项目
中央支持地方高校改革发展资金人才培养项目(2021ZYGLG001) （2021ZYGLG001）
黑龙江省自然科学基金面上项目(H 2015026) （H 2015026）
