世界中医药2025，Vol.20Issue(2)：189-197,9.DOI:10.3969/j.issn.1673-7202.2025.02.002
姜附复萎颗粒治疗脾肾阳虚型慢性萎缩性胃炎的药理作用机制研究
Pharmacological Mechanism of Jiangfu Fuwei Granules in the Treatment of Chronic Atrophic Gastritis with Spleen and Kidney Yang Deficiency Syndrome
摘要
Abstract
Objective:To explore the mechanism of Jiangfu Fuwei Granules(JFFW)in treating chronic atrophic gastritis(CAG)of spleen-kidney yang deficiency type based on network pharmacology,molecular docking,and in vivo experimental validation.Meth-ods:Network pharmacology and molecular docking techniques were employed to predict the potential targets of JFFW in CAG inter-vention,followed by validation through animal experiments.Fifty SPF-grade male SD rats were randomly divided into a blank group(n=10)and a modeling group(n=40).The CAG model was induced in the modeling group using N-methyl-N'-nitro-N-nitrosog-uanidine(MNNG),Shengdahuang Granules,and intermittent fasting.After successful modeling,the rats were randomly assigned in-to three groups:model group,Western medicine group,and Chinese medicine group according to a random number table,with 10 rats in each group.They were administered distilled water,folic acid tablets+rebamipide tablets,or JFFW by gavage for 12 weeks.Hematoxylin-eosin(HE)staining,enzyme-linked immunosorbent assay(ELISA),real-time PCR,and Western blot were used to as-sess relevant indicators.Results:Network pharmacology and molecular docking analysis identified tumor necrosis factor(TNF),Caspase-3,and Caspase-8 as core targets of JFFW in CAG intervention.Experimental results demonstrated that JFFW significantly improved gastric mucosal pathology and increased serum levels of gastrin-17(GAS-17),pepsinogen Ⅰ(PG Ⅰ),and the PG Ⅰ/PGⅡ ratio in CAG rats(P＜0.05).Furthermore,JFFW significantly downregulated mRNA and protein expression of tumor necrosis factor-alpha(TNF-α),tumor necrosis factor receptor 1(TNFR1),and TNF receptor-associated death domain protein(TRADD)in gastric tissues while upregulating the expression of Fas-associated death domain protein(FADD),Caspase-8,and Caspase-3(P＜0.05).Conclusion:JFFW alleviates or reverses CAG by regulating the TNF signaling pathway,reducing inflammation,and promo-ting apoptosis,thereby exerting anti-tumor effects.关键词
网络药理学/分子对接/慢性萎缩性胃炎/姜附复萎颗粒/TNF信号通路/扶阳流派/升举三阴法/机制研究Key words
Network pharmacology/Molecular docking/Chronic atrophic gastritis/Jiangfu Fuwei Granules/TNF signaling path-way/Fuyang School/Ascending the Three Yin Method/Mechanism分类
医药卫生引用本文复制引用
尤旭颖,蔡悦青,张月林,王坤宁,白亚丽,景燕燕,姜莉云,袁红霞..姜附复萎颗粒治疗脾肾阳虚型慢性萎缩性胃炎的药理作用机制研究[J].世界中医药,2025,20(2):189-197,9.基金项目
国家自然科学基金青年科学基金项目(82204962) （82204962）
云南省万人计划-名医专项(YNWR-MY-2018-057) （YNWR-MY-2018-057）
云南省科技厅科技计划项目基础研究计划中医联合专项-青年项目(202001AZ070001-099) （202001AZ070001-099）
天津市卫生健康委员会天津市中医药重点领域科研项目(2021006) （2021006）
天津市教委社会科学重大项目(2021JWZD23) （2021JWZD23）
