世界中医药2025，Vol.20Issue(2)：231-238,8.DOI:10.3969/j.issn.1673-7202.2025.02.007
基于肠道菌群探讨柴芍六君汤调节慢性萎缩性胃炎胃黏膜屏障的作用机制
Mechanism of Chaishao Liujun Decoction in Regulating Gastric Mucosal Barrier in Chronic Atrophic Gastritis Based on Intestinal Flora
摘要
Abstract
Objective:To explore the mechanism by which Chaishao Liujun Decoction regulates the gastric mucosal barrier in chro-nic atrophic gastritis(CAG)through 16S rDNA sequencing.Methods:Thirty-six Wistar rats were randomly divided into a normal group(n=8)and a modeling group(n=28).After successful modeling,the rats were further divided into a model group,a te-prenone group,and a Chaishao Liujun Decoction group,with eight rats in each group.The normal and model groups received sterile drinking water by gavage,while the other groups received their respective treatments for four weeks.Macroscopic indicators and gas-tric mucosal morphology were observed,and serum levels of interleukin-1 β(IL-1β),IL-18,IL-32,and gastrin-17(GAS-17)were measured.The mRNA expression levels of nitric oxide(NO),endothelial nitric oxide synthase(eNOS),trefoil factor 2(TFF2),and prostaglandin E2(PGE2)in gastric mucosal tissue were also analyzed.Additionally,16S rDNA sequencing was used to assess intes-tinal flora composition,and correlations between microbiota and barrier-protecting factors were examined.Results:Compared with the model group,the Chaishao Liujun Decoction and teprenone groups showed improvements in macroscopic indicators and gastric mucosal morphology,along with reduced levels of IL-1 β,IL-18,IL-32,GAS-17,and PGE2 mRNA expression(P＜0.01,P＜0.001).Meanwhile,NO,eNOS,and TFF2 mRNA expression levels increased(P＜0.05,P＜0.01).The teprenone group exhibited a decrease in Aliikangiella and Prevotella abundance,while the Chaishao Liujun Decoction group showed a reduction in Lactobacil-lus and Paraprevotella abundance(P＜0.05,P＜0.01,P＜0.001).Lactobacillus and Aliikangiella were negatively correlated with NO,eNOS,and TFF2 mRNA levels but positively correlated with PGE2 mRNA levels.Conversely,Desulfovibrio and Akkermansia were positively correlated with NO,eNOS,and TFF2 mRNA levels but negatively correlated with PGE2 mRNA levels(P＜0.05).Conclusion:Chaishao Liujun Decoction may restore intestinal flora homeostasis by modulating microbial abundance,thereby reduc-ing gastric mucosal inflammation and protecting the gastric mucosal barrier.关键词
慢性萎缩性胃炎/柴芍六君汤/肝郁脾虚证/肠道菌群/黏膜屏障/炎症介质/中医/大鼠Key words
Chronic atrophic gastritis/Chaishao Liujun Decoction/Liver depression and spleen deficiency syndrome/Intestinal flora/Mucosal barrier/Inflammatory factors/Traditional Chinese medicine/Rat分类
医药卫生引用本文复制引用
罗文谦,齐景馨,黄凯悦,洪银洁,甘慧娟..基于肠道菌群探讨柴芍六君汤调节慢性萎缩性胃炎胃黏膜屏障的作用机制[J].世界中医药,2025,20(2):231-238,8.基金项目
国家自然科学基金面上项目(81873237) （81873237）
福建省自然科学基金项目(2021J01929) （2021J01929）
福建省教育厅科研项目(JAT210137) （JAT210137）
第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]239号) （国中医药人教函[2022]239号）
