世界中医药2025，Vol.20Issue(2)：239-245,251,8.DOI:10.3969/j.issn.1673-7202.2025.02.008
芪黄益肺合剂调控气道炎症治疗慢性阻塞性肺疾病的作用机制
Mechanism of Qihuang Yifei Mixture in Treatment of Chronic Obstructive Pulmonary Disease by Regulating Airway Inflammation
摘要
Abstract
Objective:To observe the effect of Qihuang Yifei Mixture on airway inflammation and the NF-κB signaling pathway in mice with chronic obstructive pulmonary disease(COPD),and to explore its mechanism of action.Methods:C57BL/6 mice were randomly divided into six groups according to a random number table,with 10 mice per group.The COPD model was induced using a smoke inhalation combined with intratracheal instillation of lipopolysaccharide(LPS).After 28 days of drug intervention,lung function was measured using an animal lung function meter.Hematoxylin-eosin(HE)staining was used to observe lung tissue mor-phology and airway inflammation score.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of tumor necro-sis factor-α(TNF-α),interleukin-1 β(IL-1 β),CXC chemokine ligand 1(CXCL1),and CXC chemokine ligand 2(CXCL2).Real-time quantitative PCR(RT-qPCR)was used to assess the mRNA expression levels of TNF-α,IL-1 β,CXCL1,and CXCL2.Western blot was used to measure the protein expression of phosphorylated NF-κB p65(p-NF-κB p65)and phosphorylated inhibitor of NF-κB α(p-IκBα).Results:Compared with the normal group,the model group showed a decrease in forced expiratory volume in 0.1 second(FEV0.1),peak expiratory flow(PEF),and maximal mid-expiratory flow(MMEF)(P＜0.05),an increase in TNF-α,IL-1 β,CXCL1,and CXCL2 levels(P＜0.05),an increase in TNF-α,IL-1 β,CXCL1,and CXCL2 mRNA expression(P＜0.05),and an increase in p-NF-κB p65 and p-IκBα protein expression(P＜0.05).Compared with the model group,the high-and medium-dose Qihuang Yifei Mixture groups showed an increase in FEV0.1,PEF,and MMEF(P＜0.05),a decrease in TNF-α,IL-1 β,CX-CL1,and CXCL2 levels(P＜0.05),a reduction in TNF-α,IL-1 β,CXCL1,and CXCL2 mRNA expression(P＜0.05),and a reduc-tion in p-NF-κB p65 and p-IκBα protein expression(P＜0.05).Conclusion:Qihuang Yifei Mixture can reduce airway inflamma-tion in COPD,and its mechanism may involve inhibiting the activation of the NF-κB signaling pathway,downregulating the mRNA expression of TNF-α,IL-1β,CXCL1,and CXCL2,and reducing the chemotaxis and accumulation of downstream inflammatory cells.关键词
慢性阻塞性肺疾病/芪黄益肺合剂/气道炎症/核因子κB/炎症介质/趋化因子/肺功能/作用机制Key words
Chronic obstructive pulmonary disease/Qihuang Yifei Mixture/Airway inflammation/Nuclear factor kappa B/In-flammatory mediators/Chemokines/Lung function/Mechanism of action分类
中医学引用本文复制引用
陈斌,杨春艳,铁明慧,秦冬冬,景海卿,邢立威,李邵菲..芪黄益肺合剂调控气道炎症治疗慢性阻塞性肺疾病的作用机制[J].世界中医药,2025,20(2):239-245,251,8.基金项目
国家自然科学基金青年科学基金项目(82274374) （82274374）
云南省科技厅-云南中医药大学应用基础研究联合专项资金项目(202101AZ070001-094) （202101AZ070001-094）
云南省科技厅-云南中医药大学应用基础研究联合专项资金青年项目(202101AZ070001-153) （202101AZ070001-153）
