世界中医药2025，Vol.20Issue(2)：246-251,6.DOI:10.3969/j.issn.1673-7202.2025.02.009
基于内质网应激探讨雷公藤多苷治疗IgA肾病作用机制
Mechanism of Tripterygium wilfordii Glycosides in the Treatment of IgA Nephropathy Based on Endoplasmic Reticulum Stress
摘要
Abstract
Objective:To investigate the effect of Tripterygium wilfordii glycosides(GTW)on the endoplasmic reticulum stress-au-tophagy pathway in rats with IgA nephropathy(IgAN).Methods:Forty-five male Sprague-Dawley(SD)rats were acclimated for 1 week and then randomly assigned into four groups:blank group,model group,prednisone group,and GTW group.Except for the blank group,the remaining groups were used to establish the IgAN model by administering a combination of bovine serum albumin,lipopolysaccharide,and carbon tetrachloride.From the 13th week,the intervention groups were given oral administration of their re-spective treatments for 4 weeks.After the intervention,24-hour urine,urine routine,blood,and kidney tissue were collected.Bio-chemical tests included measurements of 24-hour urine total protein(24 h-UTP),urine red blood cell count,serum creatinine(SCr),blood urea nitrogen(BUN),and blood albumin(ALB).Hematoxylin-eosin(HE)staining was used to observe kidney pa-thology.Immunofluorescence determined the deposition of immunoglobulin IgA in the mesangial area of the glomerulus.Protein and mRNA expression levels of protein kinase RNA-like ER kinase(PERK),activating transcription factor(ATF)4,and CCAAT/en-hancer-binding protein homologous protein(CHOP)were detected by Western blot and real-time polymerase chain reaction(PCR).Results:Compared with the blank group,the model group showed significantly increased 24 h-UTP,urine red blood cell count,SCr,and BUN,along with a decreased ALB(P＜0.01).The mRNA and protein expression of PERK,ATF4,and CHOP was significantly elevated(P＜0.01).The kidney tissue had severe lesions,and IgA deposition in the kidney was prominent.Compared with the model group,the intervention groups showed significantly reduced 24 h-UTP,urine red blood cell count,SCr,and BUN,and elevat-ed ALB(P＜0.01).The mRNA and protein expression of PERK,ATF4,and CHOP was significantly reduced(P＜0.01),and kid-ney pathology and IgA deposition improved significantly.Conclusion:GTW may protect kidney function and alleviate kidney patho-logical damage in IgAN rats by regulating the PERK/ATF4/CHOP pathway,inhibiting endoplasmic reticulum stress,and improving the above biochemical indicators.关键词
IgA肾病/雷公藤多苷/内质网应激/系膜增生/免疫荧光/实验研究/作用机制/蛋白激酶R样内质网激酶/转录活化因子4/CCAAT增强子结合蛋白同源蛋白Key words
IgA nephropathy/Tripterygium wilfordii glycosides/Endoplasmic reticulum stress/Mesangial hyperplasia/Immuno-fluorescence/Experimental research/Mechanism/PERK/ATF4/CHOP分类
中医学引用本文复制引用
宋珂,宋纯东,宋丹,张守琳,丁樱..基于内质网应激探讨雷公藤多苷治疗IgA肾病作用机制[J].世界中医药,2025,20(2):246-251,6.基金项目
国家自然科学基金面上项目(82074493)——基于"肠-肾轴"探明肾必宁颗粒治疗IgA肾病的分子生物学机制 （82074493）
河南省中医药学科领军人才项目(豫卫中医函[2021]8号) （豫卫中医函[2021]8号）
河南省卫生健康委国家中医临床研究基地科研专项项目(2022JDZX003) （2022JDZX003）
河南省中医药科学研究重大专项项目(2023ZYZD02) （2023ZYZD02）
河南省"双一流"创建工程项目(HSRP-DFCTCM-2023-3-07) （HSRP-DFCTCM-2023-3-07）
