天津科技大学学报2025，Vol.40Issue(2)：37-43,7.DOI:10.13364/j.issn.1672-6510.20240039
一株PHB降解菌株的分离及其PHB降解特性研究
Isolation of a PHB Degrading Bacterium Strain and Characterization of PHB Degradation Conditions
王玉蝶 1高美荣 1马颖超 1隋丽英1
作者信息
- 1. 天津科技大学海洋与环境学院,天津 300457
- 折叠
摘要
Abstract
In our present study,a poly-3-hydroxybutyrate(PHB)-degrading bacteria strain W7 was screened from a marine fish recirculating aquaculture system.The taxonomy identification was then performed by combining its morphology,physiological and biochemical characteristics,as well as 16S rDNA sequence alignment and phylogenetic tree analysis,and strain W7 was identified as Thalassolituus.The effects of initial pH and salinity of culture medium and incubation tempera-ture on PHB degradation rate were finally investigated by inoculating strain W7 into liquid medium containing PHB as a sole carbon source.The results showed that strain W7 was identified as Gram-negative bacteria,with colorless and transparent colony and curved or rods-shaped cell without flagella.Strain W7 could utilize carbon sources such as β-hydroxy-D,L-butyric acid and L-lactic acid,and grow in salinity range of 10-50,28℃and pH 5-8.Strain W7 belonged to genus Thalas-solituus which exhibited 98.75%similarity with T.alkanivorans TMPB967(OK489464).This is the first documented Tha-lassolituus strain within this genus that exhibits the capacity of PHB degradation.The pH of medium decreased to approxi-mately 5.5 after one day incubation,while the relative PHB depolymerase activity reached its peak at day 3 except for that at salinity 50 which peaked at day 4.The highest PHB degradation rate was achieved after 5-day incubation at 28℃,pH 7.2 and salinity 30,reaching(79.70±0.52)%.In summary,our study identified strain W7 belonging to Thalassolituus with high PHB degradation capacity,which supports the applications of this strain in PHB biodegradation in marine environments.关键词
聚-3-羟基丁酸酯(PHB)/生物降解/深海弯曲菌属/PHB降解率/PHB解聚酶相对酶活力Key words
poly-3-hydroxybutyrate(PHB)/biodegradation/Thalassolituus/PHB degradation rate/relative enzyme activity PHB depolymerase分类
生物科学引用本文复制引用
王玉蝶,高美荣,马颖超,隋丽英..一株PHB降解菌株的分离及其PHB降解特性研究[J].天津科技大学学报,2025,40(2):37-43,7.
