中国组织工程研究2025，Vol.29Issue(35)：7481-7489,9.DOI:10.12307/2025.991
川续断皂苷Ⅵ促进低氧环境下MC3T3-E1细胞的成骨分化
Asperosaponin Ⅵ promotes osteogenic differentiation of MC3T3-E1 cells under hypoxia environment
李蕴喆 1牛泽蕃 1王紫瑈 2艾崇毅 2陈刚 3王新兴2
作者信息
- 1. 天津医科大学口腔医学院,天津市 300041||军事科学院军事医学研究院,北京市 100000
- 2. 军事科学院军事医学研究院,北京市 100000
- 3. 天津医科大学口腔医学院,天津市 300041
- 折叠
摘要
Abstract
BACKGROUND:Asperosaponin Ⅵ has good osteogenic effects,but its ability to promote cellular osteogenesis under hypoxia environment is not yet clear.
OBJECTIVE:To investigate the effect and potential mechanism of Asperosaponin Ⅵ on osteogenic differentiation of MC3T3-E1 cells under hypoxia environment.
METHODS:MC3T3-E1 cells were divided into three groups.Cells in the control group were cultured in a complete medium under normoxic conditions(volume fraction of 21％O2);cells in the hypoxia group were cultured in the complete medium under hypoxia conditions(volume fraction of 0.5％O2);and cells in the Asperosaponin Ⅵ group were cultured in the complete medium containing Asperosaponin Ⅵ under hypoxia conditions(volume fraction of 0.5％oxygen).After 24 hours of culture,cell counting kit-8 method and EdU staining were used to detect cell proliferation activity,TUNEL staining and western blot assay were performed to detect cell apoptosis,and flow cytometry was used to detect intracellular reactive oxygen species levels and cell cycle distribution.Each group of cells was cultured in an osteogenic induction medium containing 1×10-5,1×10-6,and 1×10-7 mol/L Asperosaponin Ⅵ under hypoxia conditions for 7 days.The optimal concentration of Asperosaponin Ⅵ for intervention was identified using alkaline phosphatase staining under optical microscopy.Western blot was used to detect the expression of bone morphogenetic protein 2,osteopontin,and PI3K/AKT signaling axis-related proteins.
RESULTS AND CONCLUSION:(1)Compared with the control group,the proliferation ability of MC3T3-E1 cells decreased under hypoxia conditions.1×10-6 mol/L Asperosaponin Ⅵ could significantly improve the cell proliferation ability under hypoxia conditions and reduce cell apoptosis.(2)Compared with the hypoxia group,the Asperosaponin Ⅵ group showed a decrease in intracellular reactive oxygen species and a significant increase in the proportion of cells in the S phase.(3)Compared with the hypoxia group,the cell morphology in the 1×10-6 mol/L Asperosaponin Ⅵ group was elongated,with more protrusions and darker alkaline phosphatase staining.(4)Compared with the hypoxia group,the expression of bone morphogenetic protein 2 and osteopontin increased in the Asperosaponin Ⅵ group,while the expression of p-PI3K and p-AKT proteins decreased.These findings indicate that under hypoxia conditions,Asperosaponin Ⅵcan promote osteogenic differentiation of MC3T3-E1 cells,possibly by regulating the PI3K/AKT pathway.关键词
MC3T3-E1细胞/低氧/川续断皂苷Ⅵ/PI3K/AKT/通路/细胞分化/成骨Key words
MC3T3-E1 cell/hypoxia/Asperosaponin Ⅵ/PI3K/AKT/pathway/cell differentiation/osteogenesis分类
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李蕴喆,牛泽蕃,王紫瑈,艾崇毅,陈刚,王新兴..川续断皂苷Ⅵ促进低氧环境下MC3T3-E1细胞的成骨分化[J].中国组织工程研究,2025,29(35):7481-7489,9.
