蒙药蓝刺头对血管内皮细胞增殖和血管生成能力的潜在作用机制
Mechanism of Mongolian medicine Echinops sphaerocephalus L.in proliferation and angiogenesis of vascular endothelial cells
摘要
Abstract
BACKGROUND:Mongolian medicine Echinops sphaerocephalus L.is a commonly used medicine for bone injury in Mongolian medicine.It is effective for tendon injury,fracture,bone nonunion,bone fever,tingling,sore and other diseases.Our previous studies have confirmed that Mongolian medicine Echinops sphaerocephalus L.can promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells,but its effect on angiogenesis in the process of bone defect repair is unknown. OBJECTIVE:To investigate the effect of Echinops sphaerocephalus L.on in vitro angiogenesis in human umbilical vein vascular endothelial cells and to explore the angiogenesis-promoting active ingredients and their mechanisms of action of Echinops sphaerocephalus L.using network pharmacology technology. METHODS:The ethanol extract of Echinops sphaerocephalus L.was prepared and preserved by freeze-drying.The proliferation,migration,chemotaxis and angiogenesis of human umbilical vein endothelial cells were observed after treatment with different concentrations(1 000,100,and 10 μg/mL)of Echinops sphaerocephalus L.The active components and possible signaling pathways that promoted angiogenesis were enriched and analyzed by network pharmacology. RESULTS AND CONCLUSION:(1)The effect of Echinops sphaerocephalus L.on angiogenesis was regulated by its mass concentration:at low mass concentration(10 μg/mL),Echinops sphaerocephalus L.could promote the proliferation,migration,chemotaxis and angiogenesis of human umbilical vein vascular endothelial cells;on the contrary,Echinops sphaerocephalus L.inhibited the proliferation,migration,and chemotaxis of human umbilical vein endothelial cells at high mass concentration(1 000 μg/mL).However,the inhibitory effect of Echinops sphaerocephalus L.on angiogenesis was not significant at high mass concentration due to the limitation of experimental time.10 μg/mL Echinops sphaerocephalus L.could up-regulate the mRNA expression of angiogenesis-associated factors,including kinase insert domain receptor,vascular endothelial growth factor A,and hypoxia-inducible factor α,and thereby influenced angiogenesis during bone repair.(2)Network pharmacological analyses indicated that Echinops sphaerocephalus L.may bind to eight core targets(TGFB1,TNF,IL-6,STAT3,CTNNB1,IL-1B,AKT1,and HIF-1A)through four core active components(apigenin,caffeic acid,quercetin,and chlorogenic acid)to exert an effect on angiogenesis,atherosclerosis,multiple viral infections,and tumor angiogenesis-related signaling pathways.关键词
蓝刺头/人脐静脉血管内皮细胞/血管生成/细胞增殖/网络药理学/工程化组织构建Key words
Echinops sphaerocephalus L./human umbilical vein endothelial cells/angiogenesis/cell proliferation/network pharmacology/engineered tissue construction分类
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方源,特木日巴根,钱智勇,何源哈达,王海燕,沙丽蓉,李筱贺,刘婧,贺雅超,张凯..蒙药蓝刺头对血管内皮细胞增殖和血管生成能力的潜在作用机制[J].中国组织工程研究,2025,29(35):7519-7528,10.基金项目
内蒙古自治区卫生健康科技项目(202201188),项目负责人:王海燕 （202201188）
内蒙古自治区高等学校创新团队发展计划项目(NMGIRT2419),项目负责人:王海燕 （NMGIRT2419）
内蒙古自治区高等学校创新团队发展计划项目(NMGIRT2227),项目负责人:李筱贺 （NMGIRT2227）
2021年内蒙古自治区蒙医药协同创新中心科学研究项目(MYYXTYB202104),项目负责人:李筱贺 （MYYXTYB202104）
内蒙古医科大学2021年度校级科研重点项目(YKD2021ZD001),项目负责人:李筱贺 （YKD2021ZD001）
2021年乌兰察布市科技基础研究项目(2021JC321),项目负责人:张凯 （2021JC321）
内蒙古自然科学基金项目(2022MS08051),项目负责人:特木日巴根 Inner Mongolia Health Science and Technology Project,No.202201188(to WHY) （2022MS08051）
Inner Mongolia Higher Education Innovation Team Development Plan,Nos.NMGIRT2419(to WHY)and NMGIRT2227(to LXH) （to WHY）
Scientific Research Project of Mongolian Medicine Collaborative Innovation Center of Inner Mongolia Autonomous Region in 2021,No.MYYXTYB202104(to LXH) （to LXH）
Key Research Project of Inner Mongolia Medical University in 2021,No.YKD2021ZD001(to LXH) （to LXH）
2021 Ulanqab Science and Technology Basic Research Project,No.2021JC321(to ZK) （to ZK）
Inner Mongolia Natural Science Foundation Project,No.2022MS08051(to Temribagen) （to Temribagen）
