畜牧与兽医2025，Vol.57Issue(5)：24-30,7.
海门山羊MSTN基因克隆、生物信息学分析及单碱基编辑的研究
Cloning,bioinformatics analysis and single base editing of MSTN gene in Haimen goat
摘要
Abstract
In order to investigate the expression of the myostatin(MSTN)gene and the structural characteristics of its encoded protein in different tissues of Haimen goat,the CDS sequence of MSTN gene of Haimen goat was cloned and the biological characteristics were analyzed.The expression level of the MSTN gene in different organs and muscle tissues of Haimen goat was checked by RT-qPCR.Three sgRNA plas-mids targeting the second and third exons of the MSTN gene were designed and constructed,respectively,and co-transfected with YE 1-BE3-FNLS into the fetal fibroblasts cell in Haimen goat.After 48 h of screening,cell DNA was extracted,and target site sequences were amplified by PCR for Sanger sequencing and TA cloning analysis.The data indicated that the CDS sequence for the MSTN gene of Haimen goat encoding 375 amino acids,which was highly conserved.The MSTN gene was expressed in different tissues and organs of Haimen goats,and the expression level of MSTN was the highest in the triceps brachii.Three sgRNA successfully introduced stop codons with editing effi-ciencies of 25％,37％,and 29％,respectively.These data laid a foundation for the use of single base gene editing technology to breed new strains of Haimen goats with MSTN gene mutation.关键词
海门山羊/肌肉生长抑制素/单碱基编辑Key words
Haimen goat/myostatin/single base editing分类
畜牧业引用本文复制引用
夏潇潇,王佳琳,李玲,佰伍加,宋志贤,岳恒,曹少先,袁玉国..海门山羊MSTN基因克隆、生物信息学分析及单碱基编辑的研究[J].畜牧与兽医,2025,57(5):24-30,7.基金项目
江苏省种业振兴"揭榜挂帅"项目(JBGS[2021]025) （JBGS[2021]025）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
高等学校学科创新引智计划项目(D18007) （D18007）
扬州市市校合作专项(YZ2023205) （YZ2023205）
