畜牧与兽医2025，Vol.57Issue(5)：43-48,6.
枯草芽胞杆菌肽聚糖介导绵羊瘤胃上皮细胞β-防御素表达的通路研究
Studies on the signaling pathway of the expression of ORECs β-defensin mediated by Bacillus subtilis peptidoglycan
摘要
Abstract
This study aimed to investigate the signaling pathways through which peptidoglycan derived from the cell wall of Bacillus subtilis induces the expression of sheep β-defensin-1(SBD-1)in ovine rumen epithelial cells(ORECs).ORECs were stimulated with 20 μg/mL peptidoglycan for 2 hours,and the mRNA and protein expression levels of SBD-1-related signaling pathway factors were analyzed using quantitative real-time PCR(RT-qPCR)and Western blot.Then,specific inhibitors were employed to block the individual signaling path-ways,followed by stimulation with 20 μg/mL peptidoglycan for 2 hours.Finally,SBD-1 mRNA and protein expression levels were measured via RT-qPCR and ELISA to identify the primary signaling pathways involved.The results showed that 20 μg/mL peptidoglycan significantly upregulated the mRNA and protein expression of TLR-2,MyD88,ERK1/2,JNK,and NF-κB(P＜0.001,P＜0.05).Inhibition of the ERK1/2,JNK,and NF-κB signaling pathways using specific inhibitors markedly reduced peptidoglycan-induced SBD-1 mRNA and protein expression in ORECs(P＜0.001,P＜0.01).These findings indicated that the induction of SBD-1 expression by Bacillus subtilis peptidogly-can was mediated through both the TLR-2-MyD88-NF-κB and TLR-2-MyD88-MAPK(JNK,ERK1/2)pathways.关键词
枯草芽胞杆菌肽聚糖/β-防御素-1/绵羊瘤胃上皮细胞/核因子κBKey words
Bacillus subtilis peptidoglycan/SBD-1/ORECs/NF-κB分类
畜牧业引用本文复制引用
齐盟,胡亮,辛雅明,白绥明,郭铸苇,杨银凤..枯草芽胞杆菌肽聚糖介导绵羊瘤胃上皮细胞β-防御素表达的通路研究[J].畜牧与兽医,2025,57(5):43-48,6.基金项目
国家自然科学基金项目(32060780) （32060780）
内蒙古自治区教育厅项目(NJZZ21015) （NJZZ21015）
