畜牧与兽医2025，Vol.57Issue(5)：49-55,7.
血管生成素在猪囊肿卵巢中的表达
Expression of angiogenin in porcine cystic ovaries
摘要
Abstract
This study aimed to investigate the localization and expression of angiogenin(ANG)in normal and cystic ovaries of pigs,and to explore the relationship between ANG expression and the occurrence of ovarian cysts.Samples of ovaries from Large White sows aged 250-300 days were collected in a slaughterhouse,and they were divided into the normal and cystic groups(n=8)based on the morphological cri-teria.Then,histological observations of normal and cystic ovaries were conducted using HE staining.Finally,Western blot was used to detect ANG protein expression in the ovaries,and immunohistochemistry was employed to detect the localization and expression of ANG in the ova-ries.The results showed that,morphologically,the cystic follicles and cystic corpora lutea were significantly enlarged,with abnormal phe-nomena such as hypertrophy of follicular theca cells and luteal cells,uneven gaps,and increased lipid droplets.The Western blot results showed that ANG expression in the cystic ovaries was significantly increased(P＜0.01).The immunohistochemistry results indicated that ANG expression was markedly elevated in theca cells of the cystic follicles and the luteal cells of the cystic corpora lutea,while the expres-sion of ANG in theca cells of the antral follicles in the normal parts of the cystic ovaries was also affected and significantly decreased.The present results indicated that there was association between ANG and the occurrence of ovarian cysts in pigs,suggesting that ANG could serve as a diagnostic marker to assist in the clinical diagnosis of ovarian cysts.关键词
血管生成素/卵巢囊肿/母猪Key words
angiogenin/ovarian cysts/sows分类
畜牧业引用本文复制引用
陆露,蔡江雪,孙一然,李平华,黄瑞华,贺斌..血管生成素在猪囊肿卵巢中的表达[J].畜牧与兽医,2025,57(5):49-55,7.基金项目
国家重点研发计划项目(2022YFD1300405) （2022YFD1300405）
国家自然科学基金项目(32372965,32072807) （32372965,32072807）
江苏高校优势学科建设工程四期项目 （）
