摘要
Abstract
To construct a heat stress model for HC Ⅱ cells,HC Ⅱ cells were divided into normothermic groups(Con),heat stress groups(HS),and heat stress-alleviation groups(HS-),and cellular activity was measured by the CCK-8 method.Cellular lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH)activity,total antioxidant capacity(T-AOC),catalase(CAT),reac-tive oxygen species(ROS),nitric oxide(NO),and malondialdehyde(MDA)contents were measured by the microplate method.The apopto-sis and cell cycle were determined by flow cytometry.The results showed that compared with the Con group,in the HS and HS-groups,cell viability was significantly decreased,LDH activity and intracellular ROS,MDA,and NO contents were significantly increased,SOD,GSH,T-AOC,and CAT activities were significantly decreased,apoptosis was significantly increased,and the cell cycle was blocked.Compared to the HS group,the HS-group showed a significant increase in cellular MDA and NO content,a significant decrease in SOD and GSH activity,and a rise in apoptosis.In conclusion,subjecting mouse mammary epithelial cells to heat stress at 42 ℃ for 3 hours,followed by recovery at 37 ℃ for 6 hours,can establish a mouse mammary epithelial cell heat stress model.关键词
乳腺上皮细胞/细胞活力/抗氧化性能/细胞凋亡Key words
HCⅡ cells/cell viability/antioxidant properties/apoptosis分类
农业科技
