西藏农业科技2025，Vol.47Issue(1)：72-77,6.
山南市藏鸡群体遗传多样性与进化关系分析
Analysis of Genetic Diversity and Evolutionary Relationship of Lhoka Xizang Chicken Population Based on Mitochondrial COX Gene Family Sequence
摘要
Abstract
This study took 25 Xizang chickens collected in the countrysides of Lhoka City and 25 Xizang chickens from the breeding farm as research materials.Focusing on the sequences of the mitochondrial cytochrome C oxidase(COX)gene family,the genetic diversity and evolu-tionary relationship of the Xizang chicken population were analyzed.Through the screening of the coding regions of the COX1,COX2,and COX3 genes,12 base mutations were discovered,including 2 non-synonymous mutations,which may have potential effects on the regulation of oxidative phosphorylation.Through the analysis of the genetic diversity of the 1 782 bp DNA fragment containing partial COX gene family,the results showed that the Pi value of 50 Lhoka Xizang chickens was 0.002 3,the K value was 4.11,and the Hd value was 0.69.In addition,the genetic information of Xizang chickens collected in countrysides was higher than that from breeding farm,indicating the resource value of the collected Xizang chickens,and effectively supplementing and improving the scale and quality of the breeding Xizang chicken population.The construction of a phylogenetic tree including subspecies of the red junglefowl showed that the Lhoka Xizang chickens were independent and intersected with the subspecies of the red junglefowl.Some Xizang chickens retained some ancient genetic information.In conclusion,this study provides a certain reference for the protection and evaluation of Xizang chicken resources.关键词
藏鸡/资源/细胞色素C氧化酶/山南Key words
Xizang chickens/resource/COX/Lhoka分类
农业科技引用本文复制引用
黄涛,王士欣,王润锦,单增桑珠,陈羲,高铭馨,巴桑,张昊,张宏林,皮劲松..山南市藏鸡群体遗传多样性与进化关系分析[J].西藏农业科技,2025,47(1):72-77,6.基金项目
国家重点研发计划项目(2022YFD1600901) （2022YFD1600901）
湖北省援疆援藏项目(2018-916-000-008) （2018-916-000-008）
山南市科技计划项目(2022-02BJKJJHXM-01). （2022-02BJKJJHXM-01）
