中国病理生理杂志2025，Vol.41Issue(4)：679-687,9.DOI:10.3969/j.issn.1000-4718.2025.04.007
巨噬细胞半乳糖型凝集素1通过抑制NF-κB信号通路延缓炎症诱导的小鼠造血干细胞分化
Macrophage galactose-type lectin 1 limits mouse hematopoietic stem cell differentiation in context of inflammation by inhibiting NF-κB signaling pathway
摘要
Abstract
AIM:To investigate the effects of macrophage galactose-type lectin 1(Mgl1)gene deletion on he-matopoietic stem/progenitor cells(HSPCs)under steady-state conditions and inflammation.METHODS:Mice were di-vided into a control group(wild-type)and an experimental group(Mgl1 gene-deleted).Flow cytometry was used to ana-lyze the proportions of various hematopoietic cell lineages in the peripheral blood and bone marrow of both groups,assess-ing the impact of Mgl1 gene deletion on steady-state hematopoiesis(n=3～4).Transplantation and colony-forming assays were utilized to evaluate the effects of Mgl1 gene deletion onthe repopulation capacity and colony-forming ability of HSPCs(n=5).The LPS-induced inflammation model was employed to examine the effects of Mgl1 gene deletion on the inflamma-tory response of HSPCs both in vitro and in vivo(n=5～8).Western blot and RT-qPCR were conducted to analyze the alter-ations in signaling pathways regulated by Mgl1 in the inflammatory response of HSPCs(n=3).RESULTS:(1)Mgl1 gene deletion had no significant effecton steady-state hematopoiesis(P>0.05).(2)Mgl1 gene deletion promoted inflam-mation-induced cell differentiation of HSPCs(P<0.01).(3)Mgl1 gene deletion accelerated the exhaustion of HSPCs un-der prolonged inflammatory conditions(P<0.01).(4)Mgl1 was found to regulate the inflammatory response of HSPCs via the NF-κB signaling pathway.CONCLUSION:Mgl1 gene deletion enhances the inflammatory response of HSPCs via the NF-κB signaling pathway.关键词
巨噬细胞半乳糖型凝集素1/造血干细胞/炎症/NF-κB信号通路Key words
macrophage galactose-type lectin 1/hematopoietic stem cells/inflammation/NF-κB signaling pathway分类
临床医学引用本文复制引用
李漫纯,詹蔷,邹密,白可,易微微,鞠振宇,陈陟阳..巨噬细胞半乳糖型凝集素1通过抑制NF-κB信号通路延缓炎症诱导的小鼠造血干细胞分化[J].中国病理生理杂志,2025,41(4):679-687,9.基金项目
国家自然科学基金青年项目(No.32300663) （No.32300663）
广东省自然科学基金面上项目(No.2022A1515011204) （No.2022A1515011204）
