中国病理生理杂志2025，Vol.41Issue(4)：688-695,8.DOI:10.3969/j.issn.1000-4718.2025.04.008
DNA损伤诱导的tsRNA在HepG2细胞中的鉴定和功能研究
Identification and functional study of DNA damage-induced tsRNAs in HepG2 cells
摘要
Abstract
AIM:To identify the expression characteristics of transfer RNA-derived small RNAs(tsRNAs)re-sponding to DNA damage in human hepatoblastoma HepG2 cells,and to investigate their potential functions.METHODS:Based on paired HepG2 cells and TP53 gene knockout HepG2 cells,we successfully constructed a DNA damage cellular model using adriamycin(ADR).Transcriptome analysis of small noncoding RNAs was performed to systematically identi-fy a set of tsRNAs responding to ADR and involved in p53 regulation.Functional enrichment analysis was conducted using the Kyoto Encyclopedia of Genes and Genomes(KEGG).Additionally,after silencing the expression of target tsRNA genes,the biological functions of these tsRNAs in HepG2 cells were initially confirmed through CCK-8 assay and plate colo-ny formation assay.RESULTS:DNA damage induced a set of tsRNAs involved in p53 regulation,among which tRF-5-1(tRF-5_tRNA-Gly-TCC-2-1)and tRF-i-1(tRF i_tRNA-Tyr-GTA-11-1)showed the most significant up-regulation in HepG2 cells(P<0.05).Silencing of either tRF-5-1 or tRF-i-1 gene inhibited the proliferative activity of HepG2 cells(P<0.05).CONCLUSION:A group of tsRNAs responding to DNA damage can be identified in HepG2 cell model,and tsRNAs can promote the proliferative activity of HepG2 cells,suggesting that tsRNAs may play an important role in the de-velopment and progression of liver malignancies.关键词
DNA损伤/转运RNA衍生的小RNA/p53蛋白/HepG2细胞Key words
DNA damage/transfer RNA-derived small RNA/p53 protein/HepG2 cells分类
临床医学引用本文复制引用
陈观子,洪甫阳,姜袁昊,张裕珍,揭育胜..DNA损伤诱导的tsRNA在HepG2细胞中的鉴定和功能研究[J].中国病理生理杂志,2025,41(4):688-695,8.基金项目
广东省自然科学基金面上项目(No.2021A1515012617) （No.2021A1515012617）
