烟草科技2025，Vol.58Issue(4)：26-33,8.DOI:10.16135/j.issn1002-0861.2024.0643
基于丁香假单胞菌基因中断技术的烟草野火病菌基因敲除
Gene knockout in Pseudomonas amygdali pv.tabaci based on Pseudomonas syringae gene disruption technique
摘要
Abstract
To establish the gene knockout technical system for Pseudomonas amygdali pv.tabaci(Pta),Pta strain D151(race 0)was used as material and plasmid pEX18Gm was selected as the starting vector to construct the pEX18Gm-HopF1-KO suicide plasmid.After conjugation transfer and several rounds of resistance screening,the effector HopF1 was knocked out in the Pta strain(race 0).The results showed that five positive mutant strains were obtained by PCR identification of the bacterial solution,and the positive transformation rate was 50％.Sequencing results further confirmed that the HopF1 gene knockout strains were successfully constructed.This study showed that the technical system of Pta gene knockout was successfully established,which lay the foundation for the cultivation of Pta-race-specialized resistant tobacco varieties and the cloning of Pta-race-specialized resistant genes in tobacco.关键词
烟草/野火病菌/效应蛋白HopF1/基因敲除Key words
Tobacco/Pseudomonas amygdali pv.tabaci/Effector HopF1/Gene knockout分类
轻工业引用本文复制引用
赫英宇,吴琳,李尊强,乔婵,万秀清,潘洪杏,郭兆奎,李若..基于丁香假单胞菌基因中断技术的烟草野火病菌基因敲除[J].烟草科技,2025,58(4):26-33,8.基金项目
黑龙江省烟草专卖局科技开发项目"抗角斑病烟草材料的创制"(2022230000200045)、"烟草野火病抗病基因定位及龙江911抗性改良"(2023230000200148). （2022230000200045）
