烟草科技2025，Vol.58Issue(4)：34-44,11.DOI:10.16135/j.issn1002-0861.2024.0885
不同青枯病抗性烤烟品种(系)根际土壤微生物群落分析
Analysis of microbial communities in rhizosphere soil for flue-cured tobacco varieties(lines)with different resistance to bacterial wilt
摘要
Abstract
To investigate the relationships between rhizosphere microbial community,soil factors and the occurrence of tobacco bacterial wilt and the resistance mechanism of flue-cured tobacco to infection by Ralstonia solanacearum,metagenomics high-throughput sequencing technology was used to study the differences in rhizosphere soil microbial community,rhizosphere soil nutrients and enzyme activities of the highly resistant strain Cuibi No.1 mutant(633K)and the highly susceptible Honghuadajinyuan(HD)under pot conditions.The results showed that the relative abundance of microbial genera such as Sphingomonas and Bradyrhizobium in 633K rhizosphere soil was higher than that in HD rhizosphere soil,while the relative abundance of Rhodanobacter and Castellaniella was higher in HD rhizosphere soil.Compared to HD,the species diversity and structural complexity of the dominant species network in the 633K rhizosphere soil decreased after Ralstonia solanacearum infection.The dominant strains with high correlations in the 633K rhizosphere soil microbial network were mainly beneficial bacteria such as Solirubrobacter pauli,Solirubrobacter soli and Edaphobacter aggregans,while the dominant strains with high correlations in the HD rhizosphere soil microbial network were mainly harmful bacteria such as Dyella jiangningensis.The contents of nitrate nitrogen(NO3--N),ammonium nitrogen(NH4+-N),microbial biomass carbon(MBC)and microbial biomass nitrogen(MBN)and urease activity(S-UE)in 633K rhizosphere soil were significantly higher than those in HD rhizosphere soil,which were 143.73％,80.05％,154.42％,44.85％and 21.50％higher than those in HD rhizosphere soil,respectively.However,acid phosphatase(S-ACP)and sucrase(S-SC)activities and organic matter(SOM)content in 633K rhizosphere soil were significantly lower than those in HD rhizosphere soil,which decreased by 47.36％,31.97％and 26.13％,respectively.Redundancy analysis showed that microbial biomass carbon was significantly correlated with microbial community structure and had the highest contribution to microbial community structure.关键词
烟草青枯病/根际土壤/微生物群落/土壤养分/土壤酶活性Key words
Tobacco bacterial wilt/Rhizosphere soil/Microbial community/Soil nutrient/Soil enzyme activity分类
轻工业引用本文复制引用
姜淑祯,冯小虎,宋文静,王卫锋,丛萍,徐艳丽,况帅,董建新..不同青枯病抗性烤烟品种(系)根际土壤微生物群落分析[J].烟草科技,2025,58(4):34-44,11.基金项目
中国农业科学院科技创新工程项目(ASTIP-TRIC03) （ASTIP-TRIC03）
中国烟草总公司江西省公司科技项目"江西烟草青枯病专性噬菌体制剂研发与应用"(2021.01.010). （2021.01.010）
