烟草科技2025，Vol.58Issue(4)：45-52,8.DOI:10.16135/j.issn1002-0861.2024.0706
湖北雪茄烟草根腐病的病原鉴定
Identification of root rot pathogen in Hubei cigar tobacco
摘要
Abstract
In order to clarify the pathogen causing root rot in Hubei cigar tobacco,diseased plant samples were collected from Danjiangkou,Enshi and Yichang regions,which are the main producing areas of Hubei cigar tobacco.The pathogen was isolated by conventional tissue isolation method,and its pathogenicity was verified by indoor inoculation of soil containing pathogen,and morphological identification and molecular biological identification were carried out.The results showed that the colonies of Fusarium on PDA medium were white to flesh-pink,and in some of them,the color deepened at a later stage and the mycelium was floccose.The conidia were typically sickle-shaped,the macroconidia were mostly septate,colorless,and sickle-shaped.Most of the microconidia were aseptate,elliptic or fusiform.Phylogenetic analysis based on ITS and EF-1 a sequences identified Fusarium oxysporum and Fusarium solani as the main pathogens causing root rot in Hubei cigar tobacco.关键词
雪茄烟草/根腐病/致病性/形态学鉴定/分子生物学鉴定/系统发育Key words
Cigar tobacco/Root rot/Pathogenicity/Morphological identification/Molecular biological identification/Phylogeny分类
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黎妍妍,杨雯婷,许汝冰,覃耀,黄俊斌,乔保明,吕培军,秦铁伟,杨勇..湖北雪茄烟草根腐病的病原鉴定[J].烟草科技,2025,58(4):45-52,8.基金项目
湖北省烟草公司重点科技项目"雪茄烟主要病虫害灾变规律调查及防控技术研究与应用"(027Y2022-023) （027Y2022-023）
中国烟草总公司重大科技项目"烟草镰刀菌根腐病成灾机制及绿色高效防控技术研发与应用"[110202401013(LS-03)]. （LS-03）
