烟草科技2025，Vol.58Issue(4)：53-62,10.DOI:10.16135/j.issn1002-0861.2024.0498
雪茄烟叶霉变生防菌株筛选及冻干菌剂制备
Screening biocontrol strain against mildew on cigar tobacco leaves and preparation of a freeze-dried bacterial agent
摘要
Abstract
To prevent and control mildew on cigar tobacco leaves in their fermentation process by biocontrol agents,an antagonistic strain with better mildew inhibitory effect was isolated from non-mildewed cigar tobacco leaves using the plate confrontation method.The fermentation conditions for the isolated antagonistic strain were optimized using a combination of single-factor experiments and response surface methodology.A biocontrol bacterial agent was prepared using vacuum freeze-drying technology,and its efficacy in preventing mould and its impact on the sensory quality of cigars were evaluated.The results showed that a biocontrol strain LK6 capable of inhibiting Aspergillus flavus was screened and identified as Bacillus amyloliquefaciens.The optimized fermentation conditions for strain LK6 were pH 7,30 ℃,rotation speed of 194 r/min,and inoculation volume of 6.5％.The freeze-dried bacterial agent was prepared with 10％sucrose as cryoprotectant,and the strain survival rate reached 82.61％.The freeze-dried agent of LK6 could effectively inhibit the growth of A.flavus even at the dilution ratio of 104,and there was no negative effect on the sensory quality of cigars by spraying the agent on cigar tobacco leaves.The stability and application efficacy of the freeze-dried agent of LK6 were satisfactory,providing a basis for using anti-mildew bioagents for cigar tobacco leaves.关键词
雪茄烟叶/霉变/生防菌株/发酵条件/冻干菌剂Key words
Cigar tobacco leaf/Mildew/Biocontrol strain/Fermentation condition/Freeze-dried bacterial agent分类
轻工业引用本文复制引用
郭紫莹,时永楠,芦柯,李宜馨,王钰,冯东民,李萌,李晓,刘琳琳,胡延奇..雪茄烟叶霉变生防菌株筛选及冻干菌剂制备[J].烟草科技,2025,58(4):53-62,10.基金项目
山东中烟工业有限责任公司科技项目"基于生物技术的雪茄烟叶防霉控霉研究"(KJXM-GS-202202006). （KJXM-GS-202202006）
