烟台大学学报（自然科学与工程版）2025，Vol.38Issue(2)：177-185,9.DOI:10.13951/j.cnki.37-1213/n.240203
自然微生物膜对刺参幼体附着变态的影响
Impacts of Natural Biofilms on Settlement and Metamorphosis of Sea Cucumber Larvae
摘要
Abstract
To investigate the influence of natural microbial biofilms on the settlement and metamorphosis of sea cu-cumber Apostichopus japonicus larvae,we analyzed variations in dry weight,density of attached bacteria,species composition,diversity and community structure of natural microbial biofilms at different ages,as well as their corre-lation with settlement selectivity and metamorphosis rate.According to the results of 16S rRNA sequencing,the community structures of microbial films varied significantly with age,and the richness and diversity of bacterial spe-cies increased dramatically with age.As biofilms matured,both the quantity and variety of bacterial species in-creased.Correlation analysis indicated a strong positive relationship(P<0.01)between settlement selectivity rates and metrics of species richness,diversity,and bacterial density.Conversely,the metamorphosis rate exhibited a significant positive correlation(P<0.05)only with indicators of species richness.These findings suggest that the age,diversity of species,and composition of the biofilm community play a significant role in influencing the settle-ment and metamorphosis processes of A.japonicus.This research provides a theoretical foundation for understand-ing the regulatory mechanisms of sea cucumber settlement and metamorphosis,as well as for advancing the cultiva-tion of high-quality seedlings.关键词
刺参/微生物膜/幼体/附着/变态/16S rRNA基因Key words
Apostichopus japonicus/biofilm/larvae/settlement/metamorphosis/16S rRNA gene分类
水产学引用本文复制引用
张立杰,赵业,王浩娜,夏雨,张燕英,孙浩,许修明..自然微生物膜对刺参幼体附着变态的影响[J].烟台大学学报（自然科学与工程版）,2025,38(2):177-185,9.基金项目
国家自然科学基金资助项目(41906098). （41906098）
