医学新知2025，Vol.35Issue(4)：386-393,8.DOI:10.12173/j.issn.1004-5511.202411181
新型纳米探针通过靶向MN受体抑制透明细胞肾细胞癌的应用研究
Application of innovative nanoprobes for targeted inhibition of clear cell renal cell carcinoma via MN receptors
摘要
Abstract
Objective To evaluate the role of novel nanomaterial MN receptor targeted inhibition system(MTIS)in targeting and inhibiting clear cell renal cell carcinoma(ccRCC).Methods The microprobe MTIS was designed and implemented for use with ccRCC cell lines 786-O and Caki-1.The targeting efficacy of both MTIS and MN was examined through laser scanning confocal microscopy(CLSM).CCK-8 and Transwell assays were conducted to confirm the inhibitory impact of MTIS on ccRCC.Additionally,the Renca cell line was utilized to induce subcutaneous tumors in mice,and MTIS was administered through tail vein injection to assess its therapeutic effect.Results The CLSM observations revealed that MTIS exhibited excellent colocalization with tumor cells.The results from CCK-8 and Transwell experiments further demonstrated that MTIS significantly inhibited the migration and invasion of tumor cells.Furthermore,the effective concentration of MTIS injected via tail vein at the tumor site in mice was observed using the in vivo imaging system(IVIS).Notably,the tumor volume in mice after 14,21 and 28 days treated with MTIS were markedly smaller compared to the control group(P＜0.05).Conclusion The novel nanoprobe MTIS effectively targets ccRCC cells and demonstrates a significant inhibitory effect on their migration and invasion.关键词
透明细胞肾细胞癌/肿瘤显影/纳米材料/靶向抑制/MN受体Key words
Clear cell renal cell carcinoma/Tumor imaging/Nanomaterials/Targeted inhibition/MN receptor分类
医药卫生引用本文复制引用
祝汉文,闫溢烁,齐鑫,李聪,王子琦..新型纳米探针通过靶向MN受体抑制透明细胞肾细胞癌的应用研究[J].医学新知,2025,35(4):386-393,8.基金项目
国家自然科学基金面上项目(82171999) （82171999）
