中国普通外科杂志2025，Vol.34Issue(3)：485-494,10.DOI:10.7659/j.issn.1005-6947.240185
m6A阅读器IGF2BP2在胰腺癌中的表达及意义
Expression and significance of m6A reader IGF2BP2 in pancreatic cancer
摘要
Abstract
Background and Aims:N6-methyladenosine(m6A)epigenetic modification plays a crucial role in post-transcriptional gene expression regulation and various physiological and pathological processes,including tumorigenesis.The m6A reader IGF2BP2 significantly enhances mRNA stability and translation efficiency and is abnormally expressed in multiple cancers.However,the specific biological function of IGF2BP2 in pancreatic cancer remains unclear.Therefore,this study investigated the expression of the m6A reader IGF2BP2 in pancreatic cancer and its effects on pancreatic cancer cell functions. Methods:The expression levels of m6A-related writers,erasers,and readers were analyzed using The Cancer Genome Atlas(TCGA),the Genotype-Tissue Expression(GTEX)database,and the Gene Expression Omnibus(GEO).Kaplan-Meier survival analysis was conducted to assess the relationship between IGF2BP2 expression and the prognosis of pancreatic cancer patients.Immunohistochemistry was used to validate IGF2BP2 expression in clinical specimens of pancreatic cancer tissues and adjacent normal tissues.Functional experiments,including CCK-8 assay,flow cytometry for cell cycle analysis,colony formation assay,and Transwell migration assay,were performed to evaluate changes in cell proliferation,cell cycle distribution,colony formation ability,and migration capacity after IGF2BP2 knockdown in pancreatic cancer cells. Results:TCGA-GTEX and GEO database analyses showed that IGF2BP2 was highly expressed in pancreatic cancer tissues(both P<0.05)and that its high expression was associated with poor overall survival(both P<0.05).Immunohistochemical staining of clinical specimens confirmed that IGF2BP2 protein expression was higher in pancreatic cancer than in adjacent normal tissue.Functional experiments demonstrated that IGF2BP2 knockdown significantly reduced the proliferation ability of pancreatic cancer cells,arrested more cells in the G0-G1 phase,decreased colony formation,and impaired cell migration(all P<0.05). Conclusion:The m6A reader IGF2BP2 is highly expressed in pancreatic cancer tissues and is closely associated with poor prognosis in patients with this disease.Its mechanism of action may be related to the promotion of cancer cell growth and migration.关键词
胰腺肿瘤/甲基化/IGF2BP2/预后/生物标记,肿瘤Key words
Pancreatic Neoplasms/Methylation/IGF2BP2/Prognosis/Biomarkers,Tumor分类
医药卫生引用本文复制引用
朱卫东,向伟,严安,欧峥嵘,朱红伟,余枭..m6A阅读器IGF2BP2在胰腺癌中的表达及意义[J].中国普通外科杂志,2025,34(3):485-494,10.基金项目
国家自然科学基金资助项目(82000614) （82000614）
湖南省自然科学基金资助项目(2021JJ70051,2024JJ5533) （2021JJ70051,2024JJ5533）
湖南省卫生健康委科研计划资助项目(202204015341) （202204015341）
中南大学湘雅三医院汇智育才基金资助项目(YX202203). （YX202203）
