中国乳业Issue(4)：84-88,5.DOI:10.12377/1671-4393.25.04.15
提取剂对酶联免疫法检测芝士粉中黄曲霉毒素M1的影响
Effect of Extracts on Determination of Aflatoxin M1 in Cheese Powde by ELISA
伍蓝 1黄华 1戴晓红 1方娟 1马后扬 1王丹 1吴建勋1
作者信息
- 1. 蒙牛乳制品武汉有限责任公司,湖北 武汉 430040
- 折叠
摘要
Abstract
[Objective]Enzyme-linked immunoassay(ELISA)was used to evaluate the effects of three different extractants of primary water,anhydrous methanol and dichloromethane on the determination of aflatoxin M1 in cheese powder.[Method]The average recovery rate was compared by comparing the relative standard deviation of extraction effects of primary water,anhydrous methanol and dichloromethane.[Result]When extracted with primary water,the relative standard deviation was 2.56%and the average recovery was 35.30%～51.12%.When extracted with anhydrous methanol,the relative standard deviation was 4.20%,and the average recovery was 81.32%～90.18%.When extracted with dichloromethane,the tissue status of the sample was abnormal,and the residue after nitrogen evaporation was oily,which could not be further determinated.[Conclusion]When extracted with anhydrous methanol.ELISA method had higher extraction accuracy and is more suitable for food determination.关键词
酶联免疫法/芝士粉/黄曲霉毒素M1/提取剂Key words
ELISA/cheese powder/Aflatoxin M1/extractant引用本文复制引用
伍蓝,黄华,戴晓红,方娟,马后扬,王丹,吴建勋..提取剂对酶联免疫法检测芝士粉中黄曲霉毒素M1的影响[J].中国乳业,2025,(4):84-88,5.
