渔业现代化2025，Vol.52Issue(2)：46-57,12.DOI:10.3969/j.issn.1007-9580.2025.02.006
淡水池塘嵌入式集装槽循环水养殖系统水质及微生物群落分析
Water quality and microbial community analysis in freshwater of pond embedded assembly tank recirculating aquaculture system
摘要
Abstract
To investigate the water quality variations and microbial community structure in the aquaculture zone of a freshwater pond with an embedded container recirculation aquaculture system,this study measured water quality indicators in the zone containing a three-stage biofilter(sedimentation tank,oxidation tank,purification tank,and filter dam)and biofill(filter cotton and brush).High-throughput sequencing was also employed to analyze the microbial community structure in the water column of each biofilter and on the biofill.Results indicate that the water purification system in the aquaculture zone operates stably and effectively,with the treated water quality meeting aquaculture requirements.The removal rates of ammonia nitrogen(NH4-N),nitrite nitrogen(NO2--N),and total nitrogen(TN)were 52.97％,63.23％,and 53.51％,respectively.The bacterial community in the aquaculture zone consisted mainly of 33 phyla,including Proteobacteria,Actinobacteria,and Bacteroidota.In the biofilter,the bacterial community structure of the biofilm differed from that of the water column,with a significantly higher relative abundance of Actinobacteria on the biofilm(P＜0.05).The water column samples had higher bacterial community diversity and richness than the biofilm samples.Correlation analysis revealed that NH4+-N,NO2--N,and TN significantly influenced the microbial community structure in the water column group(P＜0.05),while TP,AP,and DO had a more significant impact on the bacterial community structure in the biofilm group.In conclusion,the three-stage biofilter can effectively increase the microbial flora that promotes nitrogen and phosphorus metabolism,contributing to stable water quality.These findings provide theoretical support for the construction and water quality regulation of freshwater pond embedded container recirculation aquaculture systems.关键词
集装槽循环水养殖系统/生物滤池/生物膜/微生物群落/16Sr RNAKey words
assembly tank recirculating aquaculture system/biofilter/biofilm/microbial community/16Sr RNA分类
农业科技引用本文复制引用
陶志英,欧阳敏,周辉明,袁嘉欣,谢世红,贺刚,邓宏奎,李梦露,王嘉伟,邓勇辉,章海鑫..淡水池塘嵌入式集装槽循环水养殖系统水质及微生物群落分析[J].渔业现代化,2025,52(2):46-57,12.基金项目
江西省重点研发计划项目(20212BBF61009) （20212BBF61009）
南昌市特种水产繁育与健康养殖重点实验室开放基金(NCKL202203) （NCKL202203）
江西省农业农村厅农牧渔业科研指导性项目(赣农厅办函函[2023]14号-202337) （赣农厅办函函[2023]14号-202337）
江西省农牧渔业科研指导性课题(赣农厅办函[2024]12号—41) （赣农厅办函[2024]12号—41）
江西省全省现代种业(渔业)发展项目(赣财农指[2022]45号) （渔业）
