中国瓜菜2025，Vol.38Issue(4)：17-27,11.DOI:10.16861/j.cnki.zggc.2024.0684
外源H2O2结合蛭石引发对低温下无籽西瓜种子生理及转录组的影响
Effects of exogenous H2O2 combined with vermicompost initiation on physiology and transcriptome of seedless watermelon seeds at low tem-perature
摘要
Abstract
To enhance the germination performance of seedless watermelon(Heima Wangzi)under low-temperature conditions,seeds were subjected to hydrogen peroxide(H2O2)priming using concentrations of 1％,3％,5％,7％,9％,11％,13％,and 15％for 2 hours,followed by vermiculite treatment.A parallel control group was primed with distilled water for 2 hours preceding vermiculite exposure.Germination responses were assessed under controlled low-temperature stress at 15 ℃.The results demonstrated that both germination rate and germination potential initially increased and subse-quently decreased with rising hydrogen peroxide concentrations,peaking at 91％and 80％,respectively,under the 9％hydrogen peroxide treatment.These values were significantly higher than those of the control group.The activities of antioxidant enzymes,including peroxidase,superoxide dismutase,and catalase,exhibited trends similar to germination rates,with significant increases of 152.5％,157.6％,and 73.4％,respectively,compared to the control.Although malondi-aldehyde content initially peaked after vermiculite priming,it gradually declined with increasing hydrogen peroxide con-centrations.Transcriptome sequencing of seeds treated with 9％hydrogen peroxide and the control group revealed 1468 differentially expressed genes.GO analysis highlighted significant enrichment in functions related to translation,cytoplas-mic large ribosomal subunits,and ribosome structure.KEGG analysis further indicated prominent enrichment in pathways such as phenylpropanoid biosynthesis and flavonoid biosynthesis.Notably,hydrogen peroxide priming up-regulated key enzyme genes and transcription factors in the phenylpropanoid biosynthesis pathway,enhancing signal transduction and secondary metabolite synthesis,thereby improving seed adaptability and stress resistance.In conclusion,hydrogen perox-ide priming combined with vermiculite treatment effectively enhances the low-temperature germination capacity of water-melon seeds.This approach offers a practical strategy for early spring watermelon cultivation to mitigate cold stress.关键词
西瓜/蛭石引发/转录组/差异表达基因/H2O2Key words
Watermelon/Vermicompost initiation/Transcriptome/Differentially expressed gene/H2O2分类
农业科技引用本文复制引用
李灿鹏,方珂,熊程,周燕,向左芹,戴思慧,何长征..外源H2O2结合蛭石引发对低温下无籽西瓜种子生理及转录组的影响[J].中国瓜菜,2025,38(4):17-27,11.基金项目
国家西甜瓜产业技术体系(CARS-25) （CARS-25）
湖南省智能农机装备创新研发项目(ZLSS2023) （ZLSS2023）
