中国瓜菜2025，Vol.38Issue(4)：28-34,7.DOI:10.16861/j.cnki.zggc.2024.0846
西瓜EMS诱变突变体表型鉴定与矮化突变体遗传分析
Phenotypic screening of EMS mutants in watermelon and genetic dissec-tion of dwarf mutant
摘要
Abstract
The M,and M2 generation mutants induced by pollen mutagenesis of the the watermelon germplasm G42 were used as materias for investigation and statistical analysis of important agronomic traits,such as cotyledon,true leaf,flower,plant architecture,and fruit characteristics throughout the whole growth period.Among the 1244 M1 mutants,59 mutants with 18 phenotypic variations were obtained,including cotyledon color,leaf morphology,semi-dwarfism,fruit rind color,and male sterility,with a total mutation rate of 4.74％.The mutation rate for leaf-related traits was the highest at 1.12％.By screening of 9741 plants across 1007 M2 families,mutations such as semi-dwarfism,male sterility,true leaf chlorosis,and fruit size were identified,with a total mutation rate of 18.87％.The highest mutation rate was fruit traits with a level of 5.86％.Genetic analysis of the dwarf mutant M259 selected from the M2 generation revealed that the normal and dwarf plants in the F2 population constructed by crossing with G42 followed a 3∶1 segregation ratio,indicating that the dwarf trait was controlled by a recessive gene.Through the screening and identification of a large number of watermelon EMS mutant libraries,a series of mutants with variations in plant architecture,male sterility,and fruit traits were selected.These results provide valuable resources for future research on the discovery of genes related to desirable agronomic traits and the breeding of superior watermelon cultivars.关键词
西瓜/甲基磺酸乙酯/突变体筛选/遗传分析Key words
Watermelon/Ethyl methylsulfonate/Mutant screening/Genetic analysis分类
农业科技引用本文复制引用
闫梦苑,张兴平,杨路明,康旗帅,袁伟格,唐培森,豆峻岭,刘东明,杨森,朱华玉,邓云..西瓜EMS诱变突变体表型鉴定与矮化突变体遗传分析[J].中国瓜菜,2025,38(4):28-34,7.基金项目
国家自然科学基金项目(32172602) （32172602）
河南省农业良种联合攻关项目(2022010503) （2022010503）
河南省重大科技专项(221100110400) （221100110400）
