中国瓜菜2025，Vol.38Issue(4)：35-44,10.DOI:10.16861/j.cnki.zggc.2024.0353
马铃薯RBL基因家族全基因组鉴定及表达分析
Genome-wide identification and expression analysis of the potato RBL gene family
摘要
Abstract
Rhomboid-like(RBL)belongs to a conserved intramembrane serine protease,which plays an important role in plant growth and development and response to adversity stress.In this study,genome-wide identification of the potato RBL gene family was performed and gene structure,conserved motifs,cis-acting elements,chromatogram localization and gene covariance were analyzed by bioinformatics,the expression pattern was verified by combining transcriptome da-ta and qRT-PCR.The results showed that the 12 StRBL gene family members in potato were divided into five subfamilies,distributed on nine chromosomes.The gene structures and conserved motifs of the family members located in the same subfamily were basically the same;the potato RBL gene family members contained a large number of cis-acting elements related to the light response and the response to adverse stress;the results of the covariance showed that there were homol-ogous pairs of genes between potato and Arabidopsis thaliana,tomato,and chili pepper,but not in tobacco;the transcrip-tome data and qRT-PCR showed that potato RBL gene family members were highly expressed under drought stress and salt stress conditions,and there were expression differences among family members,StRBL6 and StRBL7 were up-regulat-ed under drought stress,StRBL1,StRBL7,StRBL10 and StRBL12 were significantly up-regulated under salt stress.It is speculated that RBL gene family may be involved in potato stress response.The results lay a foundation for further study on the function and mechanism of potato RBL gene.关键词
马铃薯/菱形蛋白/生物信息学/生长发育/非生物胁迫/表达分析Key words
Potato/Rhodopsin/Bioinformatics/Growth and development/Abiotic stress/Expression analysis分类
农业科技引用本文复制引用
陈啸天,满全财,汪磊,林柏松,刘晓静,孙朔,刘芳明,崔江慧..马铃薯RBL基因家族全基因组鉴定及表达分析[J].中国瓜菜,2025,38(4):35-44,10.基金项目
河北省承德市科技计划项目(202305B040) （202305B040）
河北省脱毒马铃薯繁育技术创新中心项目(SG2012016) （SG2012016）
河北(承德)马铃薯种薯产业技术研究院创新能力提升(202206F013) （承德）
