中国瓜菜2025，Vol.38Issue(4)：45-52,8.DOI:10.16861/j.cnki.zggc.2024.0577
基于KASP-SNP的辣椒品种辣美11号DNA指纹图谱构建和种子纯度检测
Application of KASP-SNP technology in fingerprint construction and seed purity detection of pepper seeds of Lamei No.11
摘要
Abstract
To provide an efficient and reliable method for the germplasm resource identification and purity detection of hybrid seeds of pepper,this study conducted genotyping of the variety Lamei No.11 and its male and female materials using kompetitive allele specific PCR(KASP)technology,which is based on 65 pairs of core single nucleotide polymor-phism(SNP)markers developed from the genomes of pepper.The genetic information of Lamei No.11 and its male and female materials were converted into two-dimensional codes by barcode online generating software,and the construction of fingerprints was completed.CaSNP10 and CaSNP11 were used to detect the purity of Lamei No.11 as 98.4％.The purity of pepper variety Lamei No.11 was also detected using simple sequence repeat(SSR)molecular marker and field identification.This study provided a theoretical basis for the protection of pepper variety rights and the identification of pepper variety purity under high-throughput genotyping.关键词
辣椒/DNA指纹图谱/品种纯度/KASP/SNP/SSRKey words
Pepper/DNA fingerprint/Cultivar purity/KASP/SNP/SSR分类
农业科技引用本文复制引用
胡宗伟,宗琛,戴蕾,魏周颖,戴祖云,杨中周..基于KASP-SNP的辣椒品种辣美11号DNA指纹图谱构建和种子纯度检测[J].中国瓜菜,2025,38(4):45-52,8.基金项目
安徽省重点研究与开发计划(2023n06020016) （2023n06020016）
